Distribuição, estrutura filogeográfica e variação genética em rodófitas continenta...
| Grant number: | 13/06436-5 |
| Support type: | Scholarships in Brazil - Doctorate |
| Effective date (Start): | June 01, 2013 |
| Effective date (End): | November 22, 2016 |
| Field of knowledge: | Biological Sciences - Botany |
| Principal Investigator: | Orlando Necchi Junior |
| Grantee: | Monica Orlandi Paiano |
| Home Institution: | Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil |
| Associated scholarship(s): | 15/01360-6 - Distribuição, estrutura filogeográfica e variação genética em rodófitas continentais brasileiras (Batrachospermales - Rhodophyta), BE.EP.DR |
Abstract The project proposes the study of populations of species of Rhodophyta, the Batrachospermales order, based on information currently available (morphological and molecular) as well as new analysis proposed here. The following hypothesis can be tested: 1) the genetic structure of population should reveal distinct pattern among species, which is related to historical biogeographical connections of each type, 2) the number of haplotypes within a single population will be small (typically 1 to 2), however a much higher number of haplotypes (typically more than 5) in all the populations of each species is found, 3) the genetic similarity among populations will progressively smaller as more far between with greater genetic diversity inter than intrapopulation for all markers used, 4) the trends of genetic divergence for each of the markers tested are congruent. To test them, the following objectives are proposed: 1) to evaluate the patterns of genetic variability of populations of several species of the order Batrachospermales (Rhodophyta) in Brazil by analyzing molecular markers of nuclear and mitochondrial genomes and also check if there is correlation between variation in the environmental and molecular data obtained, 2) determine the levels of diversity of the nuclear internal spacer (ITS), the spacer between the genes subunits 2 and 3 of cytochrome c oxidase enzyme cox the region of 2-3 and "Barcode" region cox 1 gene (encoding subunit 1 of cytochrome c oxidase); 3) estimate the intra and interpopulational genetic variability of these three molecular markers, 4) check for each species investigated the existence of levels of genetic divergence between the strains detected. The proposed project presents issues of great importance, namely: 1) provide a comprehensive systematic work involving ecological, morphological and molecular study of a representative group of continental rhodophyta to subtropical and tropical regions, 2) be innovative in Brazil for continental macroalgae and could serve as a model for future studies with other groups of rhodophyta or other divisions, and 3) in a broader sense this study will provide subsidies to comparative approaches on knowledge of the biodiversity of continental algae in tropical regions, as well as the potential for application in conservation and biomonitoring areas in aquatic environments. | |