Characterization of cells from choroid plexus using as swine animal model

• Grant number: 13/07673-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2013
• End date: May 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Carlos Eduardo Ambrósio
• Grantee: Kelly Cristine Santos Roballo
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

This project aims to characterize the cells from choroid plexus region to a greater understanding of cerebral spinal fluid production using the porcine as an animal model in its fetal stage. The collected material from porcine fetuses will be fixed in part 4% paraformaldehyde for histological analysis protocols using histology and immunohistochemistry on frozen section. And the other part will be followed by cell culture for performing the isolation, characterization and culture of the cell population using immunocytochemistry and flow cytometry techniques. The results will be analyzed and compared between the animals studied for a better comprehension of the main differences, with the goal of a greater understanding of cells present in this region in relation to their morphology and function. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROBALLO, KELLY C. S.; GONCALVES, NATALIA J. N.; PIERI, NAIRA C. G.; SOUZA, ALINE F.; ANDRADE, ANDRE F. C.; AMBROSIO, CARLOS E.. Regulation of neural stem cells by choroid plexus cells population. Neuroscience Letters, v. 626, p. 35-41, JUL 28 2016. (15/17897-9, 13/07673-0)