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Identification and phenotypic and molecular characterization of Diarrheagenic Escherichia coli strains isolated from bovine stool on confined and extensive production system

Grant number: 13/03038-9
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): September 01, 2013
Effective date (End): May 31, 2014
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal Investigator:João Pessoa Araújo Junior
Grantee:Ricardo Seiti Yamatogi
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Annually the Foodborned Diseases are response for a thousand outbreaks, hospitalization and deaths around the world, mainly in children, elderly and immunocompromised individuals, becoming a major problem for a global Public Health. The outbreaks can be caused for diverse pathogens but especial group knowledge for Escherichia coli turning a important topic in many discussion, due to the fact that E. coli is transmitted by food and its involvement in foodborne outbreaks. The main clinical manifestations caused by diarrheagenic Escherichia coli comprises the diarrhea, diarrheagenic E. coli are classified into six distinct pathotypes, considering their virulence mechanisms, the clinical syndromes, serotypes (O: H), the epidemiological aspects and type of interaction with cell lines cultured in vitro. The diarrheagenic E. coli pathotypes are: E. coli enteropatogênicas (EPEC), E. coli enterohemorrágicas (EHEC), E. coli enterotoxigenicas (ETEC), E. coli enteroinvasivas (EIEC), E. coli enteroagregativas (EAEC) e as E. coli de aderência difusa (DAEC). Animals´s products are transmitters of this agent and animals production system eventually become a major focus, presenting asymptomatic animals (reservoirs), responsible for the bacteria spread. The principal pathotypes in this animal´s food are STEC and EPEC, with a variable prevalence in several animals already investigated: poultry, swine, small ruminants and bovine, being the cattles and dairys. Currently, studies focusing EPEC and STEC are scarce in Brazil, and more studies, particularly in bovine, are really necessary. Thus the aim of this project are investigate the presence of distinct pathotypes of diarrheagenic Escherichia coli on the beef production system of confined animals and extensive, and aiming know the zoonotic potential of our isolates, to perform a phenotypic and molecular characterization evaluating the bacteria interaction with differentiated and polarized intestinal cells (Caco-2 and T84) and establishing the clonal origin of the strains obtained in the present study comparing with other diarrheagenic Escherichia coli strains previously isolated from human and food.