Control tumors induced by HPV -based immunotherapy in combination monoclonal antib...
| Grant number: | 12/25414-0 |
| Support type: | Scholarships in Brazil - Master |
| Effective date (Start): | July 01, 2013 |
| Effective date (End): | February 28, 2015 |
| Field of knowledge: | Interdisciplinary Subjects |
| Principal Investigator: | Carla Raquel Fontana Mendonça |
| Grantee: | Laura Marise de Freitas |
| Home Institution: | Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil |
Abstract The cervical cancer is a female malignant tumor that begins with intraepithelial changes of progressive nature, having HPV infection as one of the main risk factors for its emergence. The prevalence is high among young women (aged 20 to 25 years), especially in developing countries. Current therapies for the treatment of cervical cancer has been shown to be satisfactorily effective, but the biggest drawback common to all methods is the substantial excision of the cervical stroma, which can cause complications in future pregnancies. Photodynamic therapy (PDT) is proving to be a promising alternative to current treatments against many types of cancer, including cervical cancer. The technique is based on the administration of a nontoxic photosensitizing agent to the patient with subsequent exposure to a light source of predetermined wavelength. The photosensitizer (PS) selectively accumulates in tumor cells and the tissue becomes sensitive to the specific wavelength absorbed by the PS, resulting in cytotoxic response via oxidative damage. Thus, the present study aims to evaluate the efficacy of photodynamic therapy in vitro to induce cell death in cell lines infected with HPV16 (SiHa), using the photosensitizers Photogem® and methylene blue, and the association of PDT with the drug cisplatin. | |