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Microbial diversity presented on fermented juice from burned and mechanized harvesting of sugarcane and the impact on the fermentation metabolome

Grant number: 13/08431-0
Support Opportunities:Scholarships in Brazil - Doctorate
Start date: July 01, 2013
End date: July 31, 2016
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal Investigator:Carlos Alberto Labate
Grantee:Maria Leticia Bonatelli
Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

Ethanol production from sugarcane is an important renewable energy source used by automobiles in Brazil. Much technology has been developed so that the entire process - from planting to fermenting and distilling - occurred in the most efficient manner. In 2007 was established the Agroenvironmental Protocol of the Sector of Sugar and Ethanol from São Paulo which focused on deadlines for the burnt cane and implementation of mechanized harvesting. Mechanized harvesting results in different nutrient availability in the soil, which may represent a change in microorganisms present in the sugarcane soil and, therefore those who inhabit the sugarcane, as well as an increased microbial inoculum present in the leaves and stems not eliminated by burnt. As a result, there may be changes in the microorganisms present in the fermentation process, modifying the usual pattern of contaminants. Therefore, this study intends to conduct a survey of the microbiota present in stalks of sugarcane plantations of sugarcane which were or not burned, as well to follow the contaminating microorganisms present in the fermentation vats, at different stages. Finally, in an attempted to characterize the dynamics of these microorganisms in the fermentation process a transcriptomic and metabolomic analyzes will be held during fermentation in the absence and presence of commercial yeast to better understand the relationship between contaminants and the commercial yeast in the fermentation process. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BONATELLI, MARIA LETICIA; IENCZAK, JACIANE LUTZ; LABATE, CARLOS ALBERTO. Sugarcane must fed-batch fermentation by Saccharomyces cerevisiae: impact of sterilized and non-sterilized sugarcane must. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, v. 112, n. 8, p. 1177-1187, . (13/08431-0)
BONATELLI, MARIA L.; QUECINE, MARIA C.; SILVA, MARIANA S.; LABATE, CARLOS A.. Characterization of the contaminant bacterial communities in sugarcane first-generation industrial ethanol production. FEMS Microbiology Letters, v. 364, n. 17, . (13/08431-0, 11/23816-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BONATELLI, Maria Leticia. Characterization of contaminating bacterial community from ethanol fermentation process and the impact on the metabolome. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.