The controversial relationship between inflammation and prostatic lesions: treatment with Celecoxib and Goniothalamin in senile and Tramp mice

• Grant number: 13/01294-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): July 01, 2013
• Effective date (End): February 28, 2017
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Valéria Helena Alves Cagnon Quitete
• Grantee: Larissa Akemi Kido de Barros
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Senescence is associated with significant changes in the hormonal environment, causing morphological and functional changes in the prostate. Thus, the aims of this study will be evaluate the structural and molecular effects of antiinflammatory Celecoxib and Goniothalamin treatments on the ventral prostate of FVB and TRAMP mice. Also, to evaluate the IL-6, IL1²- e IL-17, TNF-2, 4HNE, inducible nitric oxide synthase, ciclooxigenase 2, do signal transducer and activator of transcription-3, nuclear factor-ºB (NF-ºB), insulin-like growth factor-1 reactivities. 72 male mice FVB and 96 TRAMP were divided into groups: Young Control (YC): 24 animals (FVB)/ 8 weeks old will be subdivided and receive carboxymethylcellulose 0,5% and PBS; Senile Control (SC): 24 animals (FVB)- 48 weeks old - the same treatment group YC. Senile Celecoxib (SNCEL): 12 animals (FVB )- 48 weeks old- 10mg/Kg/day of Celecoxib; Senil Goniothalamin (SNGTN): 12 animals (FVB) - 48 weeks old -150mg/Kg/dia of Goniothalamin; TRAMP Control 1 (T1): 24 animals (TRAMP)- 8 weeks - old will receive the same treatment group YC; Tramp Control 2 (T2): 24 animals (TRAMP) -8 weeks old- will receive the same treatment group, and will be sacrificed at 22 weeks old. Tramp 1/ Celecoxib (T1CEL): 12 animals (TRAMP) - 8 weeks old - the same treatment group SNCEL; Tramp 1/ Goniothalamin (T1GTN): 12 animals (TRAMP) -8 weeks old - the same treatment group SNGTN; TRAMP 2/Celecoxib (T2CEL) e TRAMP 2/ Goniothalamin (T2GTN): 24 animals (TRAMP), 8 weeks old - the same treatment group T1CEL e T1GTN, respectively, witch will be sacrificed at 22 weeks old. After 30 days, the ventral prostate will be collected and analyzed by morphological, Immunohistochemical, Immunoenzymatic and Western Blotting analyses. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HERMES, TULIO DE ALMEIDA; MACEDO, ALINE BARBOSA; FOGACA, ALINE REIS; RAPUCCI MORAES, LUIS HENRIQUE; DE FARIA, FELIPE MEIRA; KIDO, LARISSA AKEMI; ALVES CAGNON, VALERIA HELENA; MINATEL, ELAINE. Beneficial cilostazol therapeutic effects in mdx dystrophic skeletal muscle. Clinical and Experimental Pharmacology and Physiology, v. 43, n. 2, p. 259-267, FEB 2016. (11/02474-4, 13/17299-9, 13/01294-8)

HERMES, TULIO DE ALMEIDA; MACEDO, ALINE BARBOSA; FOGACA, ALINE REIS; RAPUCCI MORAES, LUIS HENRIQUE; DE FARIA, FELIPE MEIRA; KIDO, LARISSA AKEMI; ALVES CAGNON, VALERIA HELENA; MINATEL, ELAINE. Beneficial cilostazol therapeutic effects in mdx dystrophic skeletal muscle. Clinical and experimental pharmacology & physiology, v. 43, n. 2, p. 9-pg., 2016-02-01. (11/02474-4, 13/17299-9, 13/01294-8)

KIDO, LARISSA AKEMI; MONTICO, FABIO; VENDRAMINI-COSTA, DEBORA BARBOSA; PILLI, RONALDO ALOISE; ALVES CAGNON, VALERIA HELENA. Goniothalamin and Celecoxib Effects During Aging: Targeting Pro-Inflammatory Mediators in Chemoprevention of Prostatic Disorders. PROSTATE, v. 77, n. 8, p. 838-848, JUN 1 2017. (13/23049-5, 13/01294-8)

KIDO, LARISSA AKEMI; MONTICO, FABIO; SAUCE, RAFAEL; MACEDO, ALINE BARBOSA; MINATEL, ELAINE; VENDRAMINI COSTA, DEBORA BARBOSA; DE CARVALHO, JOAO ERNESTO; PILLI, RONALDO ALOISE; ALVES CAGNON, VALERIA HELENA. Anti-inflammatory therapies in TRAMP mice: delay in PCa progression. Endocrine-Related Cancer, v. 23, n. 4, p. 235-250, APR 2016. (13/23049-5, 13/01294-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BARROS, Larissa Akemi Kido de. Inflammation and neoplastic lesions in the prostate: chemopreventive treatments with Goniothalamin and Celecoxib in senile mice and transgenic adenocarcinoma of the mouse prostate (TRAMP). 2017. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.