Acute lung injury (ALI) is a inflammatory syndrom presenting high rate of mortality, and both inflammatory and structural pulmonary cells play a central role. In this way, epithelial cells (bronchial and alveolar) have a important role in the ALI pathophysiology. Regular aerobic training at low and moderate intensity results in protective effects in models of intra-pulmonary experimental models of ALI. Recently was described that aerobic training modulates the expression of different inflammatory mediators, fibrotic mediators and also the oxidative stress through bronchial epithelial cells in an experimental model of asthma. Therefore, the present project aims to evaluate if the protective effects of aerobic training in a model of intra and extra-pulmonary ALI results, at least partially, of direct effects of aerobic training on bronchial and alveolar epithelial cells. Thus, 6 experimental groups will be studied: control, exercise, intra-pulmonary ALI, extra-pulmonary ALI, exercise + intra-pulmonary ALI, exercise + extra-pulmonary ALI. After five weeks of aerobic training, mice will receive an intra-traqueal instilation of LPS (intra-pulmonary ALI) or an intra-peritoneal injection of LPS (extra-pulmonary ALI) and will be evaluated 24 hours after LPS administration. Total and differential cell count in bronchoalveolar lavage (BAL), citokines levels (IL-1beta, IL-6, KC, TNF-alpha, IL-10 and IL-1ra) in BAL, the number of neutrophils in the lung parenchyma will be evaluated. In another set of experiments, bronchial and alveolar epithelial cells will be isolated, and using the western blotting technique, thte expression of toll like receptos (TLRs) 2, 4, 7 and 9 will be evaluated. The TLRs are directly linked to detection and recognition of pathogens and when activated, they are responsible to coordinate or initiate the inflammatory response, aiming to protect the lungs against the pathogens. Then, the present study aims to evaluate the effects of aerobic training on the pulmonary inflammatory response induced by intra and extra-pulmonary administration of LPS, and also the effects of aerobic training on bronchial and alveolar epithelial cells, which present a central role in the development of ALI.
News published in Agência FAPESP Newsletter about the scholarship: