Diversity of amylolytic potential in filamentous fungi: purification and characterization of a glucoamylase from Aspergillus brasiliensis

• Grant number: 13/01437-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2013
• End date: April 30, 2015
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Maria de Lourdes Teixeira de Moraes Polizeli
• Grantee: Paula Zaghetto de Almeida
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Because of its territorial extension and various biomes Brazil holds among 15 to 20% of the worldwide biodiversity. This biodiversity is getting lost fast. It is estimated that only 10 % of the Brazilian species are known. Among this amount, the fungi are also known as good enzyme producers, such as amylases, that generate a great biotechnological interest. Starch is the main carbohydrate reserve in plants, and it constitutes a large part of the human population diet. Enzymes that catalyze the breakdown of starch, especially alpha-amylases, beta-amylases and glucoamylases have wide application in textile, pulp and paper, detergent, baking, pharmaceutical and beverage industries. This work aims to isolate, select and identify fungal strains, optimize fermentation processes, the purification and enzymatic characterization as well as the sequencing of at least one amylase for effective comparison of fungi from different genera or specie.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALMEIDA, PAULA ZAGHETTO; MESSIAS, JOSANA MARIA; PEREIRA, MARITA GIMENEZ; PINHEIRO, VANESSA ELISA; OLIVEIRA MONTEIRO, LUMMY MARIA; HEINEN, PAULO RICARDO; CARDOSO, GEORGE CUNHA; JORGE, JOAO ATILIO; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. Mixture design of starchy substrates hydrolysis by an immobilized glucoamylase from Aspergillus brasiliensis. BIOCATALYSIS AND BIOTRANSFORMATION, v. 36, n. 5, p. 389-395, 2018. (15/23200-0, 13/01437-3)

DE ALMEIDA, PAULA ZAGHETTO; PEREIRA, MARITA GIMENEZ; DE CARVALHO, CAIO CESAR; HEINEN, PAULO RICARDO; ZIOTTI, LUCIANA SOBRANI; MESSIAS, JOSANA MARIA; JORGE, JOAO ATILIO; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. Bioprospecção e caracterização da atividade amilolítica de fungos filamentosos da Mata Atlântica Brasileira. Biota Neotropica, v. 17, n. 3, 2017. (13/01437-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ALMEIDA, Paula Zaghetto de. Diversity of amylolytic potential in filamentous fungi: purification and characterization of a glucoamylase from Aspergillus brasiliensis. 2015. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (PCARP/BC) Ribeirão Preto.