Effect of probiotic inoculation in ovo on intestinal morphometry and Salmonella enteritidis control

• Grant number: 13/10515-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2013
• End date: February 28, 2015
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Production
• Principal Investigator: Ariel Antonio Mendes
• Grantee: Bruna Boaro Martins
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

A pretrial will be held in a hatchery of a broiler breeder company of São Paulo State, where eggs will be inoculated in the 18° day with and without probiotic, using as diluent Marek vaccine and applied through Embrex brand injections. The weights of the chicks, hatchability and mortality will be evaluated at hatching. Furthermore, there will be carried the Biometrics of 15 birds per treatment, of which 5 birds per treatment will be used for histomorphological and microbiological analysis (Salmonella Control). Treatments are as follows: T1: chicks from inoculated eggs only with the Marek vaccine, T2: chicks from inoculated eggs with Marek vaccine containing probiotic and T3: chicks from inoculated eggs only with Marek vaccine and sprayed after selection with solution containing commercial probiotic (the same used for egg inoculation). The pen floor experiment will be conducted in the experimental facilities of FMVZ-UNESP/Botucatu, using 960 males day-old Cobb ® chicks housed with a density of 12 birds/m², for 42 days. The feeding will be divided into four phases: pre-starter (1-7 days), starter: (8-21 days), growth (22-35 days) and final (36-42 days). The experimental design is a completely randomized design with three treatments and eight replicates (pens) of 40 birds each. At 7, 21, 35 and 42 days the effect of probiotic on Biometrics and Enteral Histomorphology, Scanning Electron Cecal Microscopy and Performance will be evaluated. In the experimental infection with Salmonella, 45 birds will be housed in a completely randomized design in 3 batteries with 15 chickens each, in the FMVZs Pathology Department, being used the same treatments of the floor pen experiment. The birds will be infected on the 3rd day of life through oral inoculation sample of enteric Salmonella subspecies enterica serovar Enteritidis, sorotyped and fagotyped by Adolfo Lutz Institute in São Paulo-SP, mutant resistant to nalidixic acid (Nal) and rifampicin (Rif) developed by successive crops in brilliant green agar (AVB) containing nalidixic acid (100g/ml medium) and rifampicin (100g/ml medium) as Andreatti Filho et al, 1997, to facilitate subsequent bacterial enumeration. At 7, 21 and 35 days the effect of probiotic on Biometrics and Enteral Histomorphology, Scanning Electron Microscopy of Ceco, Performance and Salmonella Control will be evaluated. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARTINS, Bruna Boaro. Efeito da inoculação de probiótico in ovo sobre a morfometria intestinal e controle de salmonella enteritidis. 2015. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.