|Support type:||Scholarships in Brazil - Scientific Initiation|
|Effective date (Start):||August 01, 2013|
|Effective date (End):||May 31, 2015|
|Field of knowledge:||Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms|
|Principal researcher:||Marcelo Brocchi|
|Grantee:||Aline Parolin Calarga|
|Home Institution:||Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil|
This study aims to evaluate the resistance profile of Salmonella entericaserovars frequently isolated in Brazilian farms during years 2002-2011. These serotypes are also commonly isolated in cases of zoonosis being a serious public health concern, since resistance of these strains may prolonger the treatment of salmonellosis. Analyses will be performed by disk diffusion method on Mueller-Hinton agar according to the Clinical and Laboratory Standards Institute. Another problem discussed in this project is related to Salmonellaidentification, seeking the presence of invA gene in serovars, which is considered as a target gene for Salmonella entericadetection. Recent studies show that this gene may not be present in all serovars, highlighting the importanceof further analyses. Extracted genomic DNA of serotypes S.Senftenberg and S. Saint Paul will be submitted to PCR for detection of the invA gene using two distinct schemes.