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Antimicrobial resistance profile in Salmonella enterica isolated from poultry and detection of invA gene in serovars Senftenberg and Saint Paul

Grant number: 13/11880-1
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2013
Effective date (End): May 31, 2015
Field of knowledge:Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
Principal researcher:Marcelo Brocchi
Grantee:Aline Parolin Calarga
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil


This study aims to evaluate the resistance profile of Salmonella entericaserovars frequently isolated in Brazilian farms during years 2002-2011. These serotypes are also commonly isolated in cases of zoonosis being a serious public health concern, since resistance of these strains may prolonger the treatment of salmonellosis. Analyses will be performed by disk diffusion method on Mueller-Hinton agar according to the Clinical and Laboratory Standards Institute. Another problem discussed in this project is related to Salmonellaidentification, seeking the presence of invA gene in serovars, which is considered as a target gene for Salmonella entericadetection. Recent studies show that this gene may not be present in all serovars, highlighting the importanceof further analyses. Extracted genomic DNA of serotypes S.Senftenberg and S. Saint Paul will be submitted to PCR for detection of the invA gene using two distinct schemes.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NOBREGA, DIEGO BORIN; CALARGA, ALINE PAROLIN; NASCIMENTO, LEANDRO COSTA; CHANDE VASCONCELOS, CARLA GASPAROTTO; DE LIMA, ELIANE MARTINS; LANGONI, HELIO; BROCCHI, MARCELO. Molecular characterization of antimicrobial resistance in Klebsiella pneumoniae isolated from Brazilian dairy herds. JOURNAL OF DAIRY SCIENCE, v. 104, n. 6, p. 7210-7224, JUN 2021. Web of Science Citations: 0.
TOMIYAMA, M. P. O.; WERLE, C. H.; MILANEZ, G. P.; NOBREGA, D. B.; PEREIRA, J. P.; CALARGA, A. P.; FLORES, F.; BROCCHI, M. Salmonella enterica Typhimurium fljBA operon stability: implications regarding the origin of Salmonella enterica I 4,[5], 12:i:-. Genetics and Molecular Research, v. 14, n. 4, p. 19057-19065, 2015. Web of Science Citations: 0.

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