Genomic studies of global gene expression of Aspergillus niger grown on sugarcane bagasse and stem

• Grant number: 13/09541-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2013
• End date: July 31, 2015
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Fábio Márcio Squina
• Grantee: Camila Cristina Sanchez
• Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Campinas , SP, Brazil
• Associated research grant: 08/58037-9 - Library generation for biomass-conversion enzymes from soil metagenome, AP.BIOEN.JP

Abstract

The dependence on fossil fuel sources as well as the concern about the environment has generated a worldwide interest in establishing new sources of fuel and energy. In this sense, the use of ethanol as a fuel is advantageous given its inexhaustible energy source and minimal environmental impact. Currently, Brazil is the world's second largest producer of ethanol, which is produced from sugar cane juice fermentation. However, several studies suggest that with the use of bagasse and straw, Brazil could double its production per hectare. Nevertheless, the use of this biomass presents a challenge, because is necessary to degrade plant cell wall structure (composed of complex sugars) into single fermentable sugar (such as glucose). To achieve this aim, several types of hydrolytic enzymes are required, that are produced by a variety of microorganisms. In this sense, Aspergillus niger is a fungus capable of producing and secreting those enzymes in large quantities. Although several studies have been performed with this microorganism, our understanding of the exact mechanisms of degradation of these substrates remains unclear. Therefore this project aims to analyze the global gene expression of A. niger cultivated on medium containing bagasse and stem from sugarcane as a substrate, by massively parallel high-throughput sequencing of cDNA (RNA-seq). Besides contributing to the understanding of biomass degradation, the knowledge on the molecular level resulting from this work will reflect on new perspectives for the development of enzyme cocktails, enabling the large-scale production of second-generation ethanol. (AU)

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

BORIN, GUSTAVO PAGOTTO; SANCHEZ, CAMILA CRISTINA; DE SANTANA, ELIANE SILVA; ZANINI, GUILHERME KEPPE; CORREA DOS SANTOS, RENATO AUGUSTO; PONTES, ANGELICA DE OLIVEIRA; DE SOUZA, ALINE TIEPPO; TAVARES SOARES DAL'MAS, ROBERTA MARIA MENEGALDO; RIANO-PACHON, DIEGO MAURICIO; GOLDMAN, GUSTAVO HENRIQUE; et al. Comparative transcriptome analysis reveals different strategies for degradation of steam-exploded sugarcane bagasse by Aspergillus niger and Trichoderma reesei. BMC Genomics, v. 18, JUN 30 2017. (11/08945-9, 15/08222-8, 14/15799-7, 13/09541-4, 14/11733-1, 14/11766-7)

BORIN, GUSTAVO PAGOTTO; SANCHEZ, CAMILA CRISTINA; DE SOUZA, AMANDA PEREIRA; DE SANTANA, ELIANE SILVA; DE SOUZA, ALINE TIEPPO; PAES LEME, ADRIANA FRANCO; SQUINA, FABIO MARCIO; BUCKERIDGE, MARCOS; GOLDMAN, GUSTAVO HENRIQUE; DE CASTRO OLIVEIRA, JULIANA VELASCO. Comparative Secretome Analysis of Trichoderma reesei and Aspergillus niger during Growth on Sugarcane Biomass. PLoS One, v. 10, n. 6, JUN 8 2015. (13/01071-9, 13/09541-4, 11/08945-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANCHEZ, Camila Cristina. Global gene expression of Aspergillus niger grown on bagasse and culm of sugarcane. 2016. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.