Advanced search
Start date
Betweenand

Analysis of chromosomal instability through the cytokinesesis-block micronucleous assay (CBMN) in myelodysplastic syndromes

Grant number: 13/13312-0
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): November 01, 2013
Effective date (End): March 31, 2014
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Maria de Lourdes Lopes Ferrari Chauffaille
Grantee:Silvia Natsuko Akutsu
Supervisor abroad: Shinya Matsuura
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Local de pesquisa : Hiroshima University, Japan  
Associated to the scholarship:12/10381-9 - Identification of chromosomal abnormalities in patients with myelodysplastic syndromes and correlation with the methylation profile and expression of genes IRF-1 and PPARgamma, BP.MS

Abstract

Myelodysplastic Syndromes (MDS) is characterized by a clonal hematopoiesis, differentiation with aberrant peripheral cytopenias, and a risk of progression to acute myeloid leukemia (AML). There are somatically acquired genetic abnormalities that lead to the main characteristics that define the SMD. The chromosomal instability is considered to be one of the mechanisms that generates abnormalities in hematopoietic cells. Micronuclei are formed from acentric chromosomal fragments or chromatic and whole chromosomes that are not incorporated into the nucleus of the daughter cell during cell division. By the fact of the presence of micronuclei be considered indicative of the prior existence of chromosomal changes, the analysis of their frequency can be proposed as an alternative to classical cytogenetic methods for monitoring chromosomal damage. One can use the detection of cytogenetic effects in subjects chronically exposed to mutagenic and/or carcinogenic and is also considered a marker of chromosomal instability in tumors. The present study aims to add information to the initial design, through analysis of chromosomal instability by micronucleus assay with cell block in cytokinesis (CBMN) in patients with MDS. One will compare the frequency of micronuclei in erythrocytes and bone marrow erythroblasts with the frequency of micronuclei in peripheral blood lymphocytes. It will also be made to correlate with the results of karyotype already undertaken and other relevant examinations. (AU)