Analysis of chromosomal instability through the cytokinesesis-block micronucleous assay (CBMN) in myelodysplastic syndromes

• Grant number: 13/13312-0
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Effective date (Start): November 01, 2013
• Effective date (End): March 31, 2014
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Maria de Lourdes Lopes Ferrari Chauffaille
• Grantee: Silvia Natsuko Akutsu
• Supervisor: Shinya Matsuura
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Research place: Hiroshima University, Japan
• Associated to the scholarship: 12/10381-9 - Identification of chromosomal abnormalities in patients with Myelodysplastic Syndromes and correlation with the methylation profile and expression of genes IRF-1 and PPARgamma, BP.MS

Abstract

Myelodysplastic Syndromes (MDS) is characterized by a clonal hematopoiesis, differentiation with aberrant peripheral cytopenias, and a risk of progression to acute myeloid leukemia (AML). There are somatically acquired genetic abnormalities that lead to the main characteristics that define the SMD. The chromosomal instability is considered to be one of the mechanisms that generates abnormalities in hematopoietic cells. Micronuclei are formed from acentric chromosomal fragments or chromatic and whole chromosomes that are not incorporated into the nucleus of the daughter cell during cell division. By the fact of the presence of micronuclei be considered indicative of the prior existence of chromosomal changes, the analysis of their frequency can be proposed as an alternative to classical cytogenetic methods for monitoring chromosomal damage. One can use the detection of cytogenetic effects in subjects chronically exposed to mutagenic and/or carcinogenic and is also considered a marker of chromosomal instability in tumors. The present study aims to add information to the initial design, through analysis of chromosomal instability by micronucleus assay with cell block in cytokinesis (CBMN) in patients with MDS. One will compare the frequency of micronuclei in erythrocytes and bone marrow erythroblasts with the frequency of micronuclei in peripheral blood lymphocytes. It will also be made to correlate with the results of karyotype already undertaken and other relevant examinations. (AU)