Microbiological evaluation of fluid pipe sonication endotracheal removed for pediatric patients in the intensive care unit and comparison with the culture of secretion resulatados tracheal

• Grant number: 13/00130-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2013
• End date: July 31, 2014
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Mauro José Costa Salles
• Grantee: Rafael Yoshio Koto
• Host Institution: Faculdade de Ciências Médicas da Santa Casa de São Paulo (FCMSCSP). Fundação Arnaldo Vieira de Carvalho. São Paulo , SP, Brazil

Abstract

Nosocomial Pneumonia, especially when associated with mechanical ventilation is one of the most common causes of death from infection in patients in the ICU. In pediatric patients under intensive care, this type of infection occurs in about 2.3 cases per 1000 hours of ventilation. The diagnosis of Ventilator-Associated Pneumonia (VAP) is based on radiological criteria associated with clinical and microbiological profiles based on samples obtained commonly by endotracheal suctioning. The secretion culture obtained by this method despite being the most widely used, has data with high sensitivity and specificity variations, subjecting to the occurrence of false positives and false negatives. Aiming to improve these data, the culture of the fluid of sonication of the endotracheal tube, the method by which the application of ultrasound detaches the layer of biofilm from the surface of the material, has emerged as a new diagnostic approach. The objective of this study is to validate the culture of the fluid of sonication of an endotracheal tube as a complementary diagnosis in patients with VAP, and comparatively evaluate the quantitative culture of tracheal secretions with sonication fluid from the biofilm attached to the endotracheal tube in the same patients diagnosed with VAP. The study is a prospective pilot of microbiological analysis and comparison between the results obtained by culture from the fluid of sonication of endotracheal tubes and cultures derived from tracheal secretions collected during the intubation, and when the withdrawal of the tube. (AU)