EcPVs DNA detection in aural plaques samples before and after imiquimod treatment

• Grant number: 13/13973-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2013
• End date: July 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: José Paes de Oliveira Filho
• Grantee: Luiza Stachewski Zakia
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The aural plaque is a cosmopolitan disease in distribution that causes discomfort to the animals and economical losses to the owners. Seven types of Equus caballus papillomavirus (EcPV) have been identified. Four of them were associated with aural plaque, EcPV3, 4, 5 e 6. Even though the lesions do not regress spontaneously, a previous study demonstrated success on treating aural plaques with imiquimod cream 5%. However, this study was performed previously the association of the EcPVs with aural plaque in horses; for that reason, any studies that detect the presence of de viral DNA after treatment have been done yet. Therefore, the aim of this study is to verify the presence of the EcPV1-7 DNA by PCR in samples of aural plaques before and after the treatment of the lesions with imiquimod, as well as to evaluate the treatment efficiency clinically.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZAKIA, LUIZA S.; OLIVO, GIOVANE; BASSO, ROBERTA M.; MIRA, JULIANA; HERMAN, MARIANA; ARAUJO, JR., JOAO P.; BORGES, ALEXANDRE S.; OLIVEIRA-FILHO, JOSE P.. Imiquimod treatment for Equus caballus papillomavirus infection in equine aural plaques. VETERINARY DERMATOLOGY, v. 27, n. 3, p. 175+, JUN 2016. (13/15995-8, 13/13973-7)

ZAKIA, L. S.; BASSO, R. M.; OLIVO, G.; HERMAN, M.; ARAUJO, JR., J. P.; BORGES, A. S.; OLIVEIRA-FILHO, J. P.. Detecção do DNA de papilomavírus em amostras de placa aural equina fixadas em formalina e embebidas em parafina. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 67, n. 4, p. 1193-1196, AUG 2015. (13/15995-8, 12/06378-2, 13/13973-7)