Microorganisms adherence on lithium disilicate ceramic surface exposed to different solutions

• Grant number: 12/18880-4
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): September 01, 2013
• Effective date (End): August 31, 2014
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal researcher: Daniela Micheline dos Santos
• Grantee: Beatriz Cristiane Zuin Monteiro
• Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The preparation finish line and the influence of some solutions is a concern in relation to ceramic restorations. We know that to achieve success and longevity with esthetic restorations it is essential to have periodontal health. Terminals that are located subgingivally can cause damage to it. Moreover, some solutions commonly used can affect the properties of some restorative materials. However, the literature is poor when it comes to the influence of such solutions in dental ceramics. The increased surface roughness caused by these solutions may facilitate adherence of some pathogenic microorganisms, causing gingival inflammation. Therefore, this study will assess the effect of different surface roughness ceramic of lithium disilicate and since this favors the adhesion of microorganisms that are involved in the pathogenesis of periodontal disease, namely: Porphyromonas gingivalis and Fusobacterium nucleatum. 108 samples will be prepared, half for each strain, with 1mm thickness and 5 mm in diameter. For each type of bacteria, the samples are randomly distributed in six groups according to the solutions to be dumped. These are: Sodium Fluoride 0.2%, FluorGel, artificial saliva, Coca-Cola, Cool Mint Listerine and hydrogen peroxide 7.5%. The readings roughness will take place in the initial period and after the immersion process for each group. After immersion will be held the microbiological assays. For this, the microorganisms are cultured on BHI agar supplemented with defibrinated horse blood. After standardization of innocuous, each species will be inoculated in BHI with the specimen inside. After 72 hours, this is removed, washed and shaken in a tube with saline to desprendimentos adhered cells. These samples are subsequently undergo serial dilutions plated to assess the amount of adhered cells, by counting the colony forming units.