Genotyping of anaerobic bacteria isolated from bovine periodontitis lesions

• Grant number: 13/13701-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2013
• End date: February 01, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Iveraldo dos Santos Dutra
• Grantee: Ana Carolina Borsanelli
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated scholarship(s): 15/06917-9 - Identification of the bacteria associated with bovine periodontitis and oral health using bacterial culture and high-throughput 16S rRNA gene sequencing, BE.EP.DR

Abstract

Bovine periodontitis ("cara inchada") is a disease characterized by a progressive process that begins between deciduous premolars teeth of the jaw of calves due to tooth eruption. Of great economic and sanitary impact in the 1960s and 1970s, associated to the formation of pastures in extensive areas of the Southeast and Midwest regions of the country, nowadays the disease occurs after retirement of grazing or feeding of cattle with fodder grown in previously endemic areas. Periodontitis afflicts up to 25 to 60% of the animals, which mostly shows no facial convexity ("cara inchada"), a fact that complicates the identification of the disease that leads to progressive weight loss of the animals, loss of teeth, stunted growth, diarrhea and death by starvation when they remain in endemic pastures. The clinical, pathological, bacteriological, epidemiological, treatment and control evidences observed the primary involvement of periodontopathogenic anaerobic microbiota, predominantly Gram-negative, in the pathogenesis of the disease. Preliminarily characterized in the 1980s as belonging to the genus Bacteroides (pigmented or not of black), Fusobacterium and Actinomyces (Gram-positive), these bacteria produce enzymes and toxins with histolytic and endotoxic capacity with primary and secondary potencial in the pathogenesis of periodontal lesions, even similar to what occurs in different animal species and man. The clinical recovery of affected animals is associated with the quantitative remission in the composition of microflora considered periodontopathogenic and occurs after the cattle transference of the endemic pasture to an indene area, in the same way seen that after the use of virginiamycin or spiramycin, administered by mineral supplementation. This study aims to identify the main anaerobic bacteria isolated from bovine periodontal lesions by polymerase chain reaction, using primers of the main periodontopathogenic bacteria.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BORSANELLI, ANA CAROLINA; GAETTI-JARDIM JUNIOR, ELERSON; DOEBEREINER, JUERGEN; DUTRA, IVERALDO S.. Treponema denticola na microbiota da periodontite bovina. Pesquisa Veterinária Brasileira, v. 35, n. 3, p. 237-240, MAR 2015. (13/13701-7)

BORSANELLI, ANA CAROLINA; GAETTI-JARDIM JUNIOR, ELERSON; SCHWEITZER, CHRISTIANE MARIE; DOEBEREINER, JUERGEN; DUTRA, IVERALDO S.. Presença de espécies de Porphyromonas e Prevotella na microbiota oral de bovinos com periodontite. Pesquisa Veterinária Brasileira, v. 35, n. 10, p. 829-834, OCT 2015. (13/13701-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BORSANELLI, Ana Carolina. Genotyping of anaerobic bacteria isolated from bovine periodontitis lesions. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.