Full text
| |
| Author(s): |
Ana Carolina Borsanelli
Total Authors: 1
|
| Document type: | Doctoral Thesis |
| Press: | Jaboticabal. 2017-03-08. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal |
| Defense date: | 2017-02-07 |
| Advisor: | Iveraldo dos Santos Dutra; Elerson Gaetti Jardim Júnior |
| Abstract | |
Bovine periodontitis is a progressive purulent infectious process associated with the presence of strict anaerobic subgingival biofilm. Seasonal and associated to soil and dietary management, the disease has variations in its clinical presentation, which includes since an aggressive form until chronic manifestations. The economic losses are significant and result from difficulties in gripping, chewing and rumination. The present study aimed to identify and characterize bovine periodontitis and identify the microorganisms associated with periodontal lesions by molecular methods. In the evaluation of the microbiota of the periodontal pocket (n=26) and gingival sulcus (n=25) of cattle, by polymerase chain reaction (PCR) and with the use of 35 primers of species of potential pathogens, it can be associate the occurrence of Actinobacillus naeslundii, Enterococcus faecium, Porphyromonas asaccharolytica, Porphyromonas endodontalis, Prevotella buccae, Prevotella intermedia, Prevotella melaninogenica, Prevotella nigrescens, Prevotella oralis, Treponema denticola and Treponema pectinovorum with bovine periodontitis. In a study carried out in Scotland to verify the occurrence of periodontal lesions in slaughtered animals, 200 dental arches were examined, of which 24 (12%) presented periodontal lesions in the incisors or masticatory teeth. This unpublished study reveals that periodontitis is not uncommon in cattle slaughtered in West of Scotland and is clearly a neglected health problem in animal production and welfare. At the opportunity, the risk factors associated with the disease were evaluated in a universe of 250 slaughtered animals, of which 35 had periodontal lesions and 40 were periodontally healthy. By the logistic regression analysis was evaluated the association between the independent variables, sex, age and race with periodontitis. The age of the animals was significantly associated with the presence of periodontal lesions. For each extra year in age, a cow is 1.53 times more likely to develop periodontitis (p<0.001). Gender was not significantly associated with periodontitis (p=0.887). Regarding the variable breed type, beef cattle were 0.36 times as likely to have periodontitis compared to dairy cattle. At the same occasion, samples of subgengival biofilm of 40 bovines with periodontitis and 38 periodontally healthy were submitted to high- throughput sequencing. In the bovine microbiome the most discriminative taxa in the samples of healthy animals were Gastranaerophilus, Planifilus, Burkholderia and Arcobacter. In animals with periodontitis, the most prevalent microorganisms were Elusimicrobia, Synergista, Propionivibrio, Fusobacteria, Wolinella, Porphyromonas, Candidatus, Prevotella, Firmicutes (uncultivable), Bacteroides and Treponema. In conclusion, the two groups of bovines evaluated harboured distinct microbial profiles, and the samples of bovines with periodontitis were more diverse in microorganisms than those of healthy cattle. In this unprecedented context, in the oral microbiology of bovines we can verify the main components in the bacterial homeostasis of the biofilm of healthy sites and the dysbiosis in the periodontal lesions, providing indicators for and evolution of the knowledge on bovine periodontitis. (AU) | |
| FAPESP's process: | 13/13701-7 - Genotyping of anaerobic bacteria isolated from bovine periodontitis lesions |
| Grantee: | Ana Carolina Borsanelli |
| Support Opportunities: | Scholarships in Brazil - Doctorate |