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Delimitation of Anemopaegma species complex (Vell.) Stellf. ex de Souza (Bignonieae, Bignoniaceae) based on transposable elements

Grant number: 13/10362-7
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): September 01, 2013
Effective date (End): August 31, 2016
Field of knowledge:Biological Sciences - Botany
Principal Investigator:Marie-Anne van Sluys
Grantee:Fabiana Firetti-Leggieri
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


The correct delimitation of species is essencial, since these represent the fundamental units for taxonomic studies and for other biological studies such as genetics, ecology, evolution, biogeography, among others. The delimitation of species is usually done through morphological characters. However, for some groups with a history of reticulate evolution, it becomes necessary to use molecular markers along with morphological data for the identification of taxa. Anemopaegma arvense species complex is composed of three species which show great morphological variation possibly generated by recurrent processes of hybridization and introgression. On the other hand, the molecular mechanisms that determine the speciation processes are poorly understood and of these we have a set of repetitive sequences of the genome called transposable elements. According to the above, this project aims to determine the transposable elements present in A. arvense, A. glaucum (parental species) and in A. acutifolium (hybrid species) from the production of genomic information coming from Next-generation sequencing and, subsequently, use these transposable elements as molecular markers for the correct delimitation of the taxa that comprise the A. arvense species complex. The specimens will be collected in the "cerrado" areas of Bahia, Goiás, Minas Gerais, São Paulo states and of Distrito Federal. DNA sequencing will be conducted with the platform 454 in the Laboratory CATG (IQ-USP) e the remaining steps are performed in the Laboratory GaTE, Department of Botany, University of São Paulo. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FIRETTI, FABIANA; ZUNTINI, ALEXANDRE RIZZO; GAIARSA, JONAS WEISMANN; OLIVEIRA, RENATA SOUZA; LOHMANN, LUCIA G.; VAN SLUYS, MARIE-ANNE. Complete chloroplast genome sequences contribute to plant species delimitation: A case study of the Anemopaegma species complex. AMERICAN JOURNAL OF BOTANY, v. 104, n. 10, p. 1493-1509, OCT 2017. Web of Science Citations: 8.

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