Construction and application of a paper-based analytical device for the diagnosis of malaria caused by Plasmodium falciparum

• Grant number: 13/09171-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): October 01, 2013
• Effective date (End): July 16, 2017
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Lauro Tatsuo Kubota
• Grantee: Glauco Pilon dos Santos
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 08/57805-2 - Institute of Bioanalytics, AP.TEM
• Associated scholarship(s): 14/18190-3 - Paper-based analytical platform for point-of-care immunodiagnostics with amplified response and automated assay steps, BE.EP.DR

Abstract

Malaria, a serious infectious disease and considered neglected, is a relevant public health problem, responsible for approximately 1 million of deaths per year. It is caused by protozoan parasites of the genus Plasmodium, being Plasmodium falciparum the most severe and potentially fatal specie, which requires an early detection of the disease in order that the appropriate treatment enables the reduction of mortality rates due to its contamination.Lateral flow immunoassay (LFIA) is a widely used paper-based diagnostic system and consists of a fast, practical and low cost test that not requires specific knowledge and equipment for its use. It is quite applied for screening and can generate qualitatively and quantitatively information about the presence of an analyte by using antibodies or antigens conjugated with any type of marker, which will be responsible for identifying the interaction between antigen and antibody by different types of detection.This work aims the construction of a paper-based analytical device for the diagnosis of malaria caused by Plasmodium falciparum by the conjugation of different markers to the antibody in order to provide an increase in the sensitivity by amplifying the signal, allowing the colorimetric, fluorescence and electrochemical detection of the HRP2 protein at the early stage of the disease. The most promising detection method for the development of a technological product, by comparative analysis of the spot tests, will be employed in a lateral flow immunoassay system. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS SANTOS, GLAUCO PILON; CORREA, CATIA CRISPILHO; KUBOTA, LAURO TATSUO. A simple, sensitive and reduced cost paper-based device with low quantity of chemicals for the early diagnosis of Plasmodium falciparum malaria using an enzyme-based colorimetric assay. SENSORS AND ACTUATORS B-CHEMICAL, v. 255, n. 2, p. 2113-2120, FEB 2018. (08/57805-2, 13/09171-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTOS, Glauco Pilon dos. Construction and application of paper-based analytical devices in the detection of biomarkers for clinical diagnosis. 2017. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Química Campinas, SP.