OBTAINMENT OF ALTERNATIVE PREBIOTIC SOURCES AND USE IN BROILER DIETS

• Grant number: 13/15481-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2013
• End date: February 29, 2016
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
• Principal Investigator: Luciana Francisco Fleuri
• Grantee: Mayara Rodrigues Pivetta
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The prebiotics-type fructan show wide degree of polymerization (DP), what can promote differences in fermentation and in their effects on the microbiota of determined organism. Considering the possible synergic effect of utilizing fructans with diverse DP and the importance in to reduce the dependence by external inputs, it become fundamental the innovation and biotechnological development on fructans production and their application. The objective of this research is: select an alternative substrate which stimulates higher growth of microorganisms that produce endo-inulinase; select microorganisms that produce endo-inulinase, extract the enzyme and apply it on the inulin and; evaluate the effect of supply of inulin treated with endo-inulinase and the solid enzymatic preparation (SEP) on the performance, cecal pH, lipid biochemical parameters, turnover on intestinal mucosa and microbial parameters in broilers. On first experiment it will be selected the best combination among the fungal strains of Aspergillus oryzae, A. niger, A. brasiliensis, A. flavipus, A. wentii, Penicillium roqueforti, P. expansum e Kluyveromyces marxianus with more 20 strains belonging to the collection of the Laboratory of Microorganisms, Institute of Biosciences at UNESP, and among the substrates wheat bran, soybean meal, bran or husk of yacon and sugarcane bagasse, which shows the highest activity of endo-inulinase using the process of solid state fermentation (SSF). It will be performed the following procedures: tracking of endo-inulinase producing fungi, production of endo-inulinase with isolated substrates, determination of enzymatic activity, kinetics of enzyme production, concentration of endo-inulinase selected, hydrolysis of inulin, obtainment of SEP and biochemical characterization. The second experiment will be carried out in a randomized block design, with four replications of 25 birds each. The treatments will be constituted by the basal diet supplementations: control (T1), growth promoter (T2), commercial inulin (T3), inulin treated with endo-inulinase (T4), inulin treated with endo-inulinase and commercial inulin (1:1) (T5) and SEP (T6). It will be evaluated: performance, cecal pH and lipid biochemical parameters. On third experiment will be evaluated the turnover on intestinal mucosa. It will be used the randomized block design, with five replications of one bird each. The birds will be divided on three experimental groups, according to the treatment adopted: T1 - control; T2 and T3 - prebiotics 1 and 2, chosen according to the results of the treatments that do not reduce the performance on second experiment. At 21 days old, the birds will be challenged with Salmonella enterica (SE). For the microbial parameters, it will be evaluated: identification and selection of Lactobacillus spp. and Bifidobacterium spp. and in vitro antimicrobial activity. For count of SE, it will be used the randomized block design in a 3x6 factorial scheme, constituted by three experimental diets and six sampling periods, with eight replications of one bird each.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PIVETTA, Mayara Rodrigues. Obtaining of alternative prebiotic sources and its use in broiler diets. 2016. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.