Phylogenetic analysis of the Vaccinia virus (VACV) circulating in wild and domestic animals of the Central West region of São Paulo State

• Grant number: 13/07693-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2013
• End date: June 30, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Jane Megid
• Grantee: Marina Gea Peres Serafim
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The vaccinia virus (VACV) is the etiologic agent of an exanthematic illness, characterized by the appearance of skin lesions on teats and udders of cows, and oral mucosa of calves. It also affects humans, who acquire the infection by direct contact with lesions during milking, developing similar events in the hands, arms and forearms. In Brazil, since the end of the massive worldwide vaccination program against smallpox Humana (VARV), the VACV presents itself as an important re-emerging disease, affecting cattle and humans causing economic loss due to the decrease in milk production and the occurrence of mastitis and other secondary bacterial infections. Although the origin of outbreaks in the country remains unknown, the most accepted theory assumes that there is a population of samples with VACV genetic and phenotypic diversity circulating in a population of natural reservoirs still unknown, and depending on the biological and geographical conditions are transmitted to hosts accidental (cows and humans). Considering the importance of VACV, the economic losses resulting from the outbreaks, the unknowns about the origin, species, reservoirs and mechanisms of viral spread, added the results previously obtained in Masters research project assistance under FAPESP 2010/07908-0, which got to 1371 samples of blood and serum, of which 274 were positive in the serum neutralization test and 67 in the PCR test, the objectives of the present study aimed to elucidate these unknowns through phylogenetic analysis of viral DNA extracted from these samples. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PERES, MARINA G.; BACCHIEGA, THAIS S.; APPOLINARIO, CAMILA M.; VICENTE, ACACIA F.; MIONI, MATEUS S. R.; RIBEIRO, BRUNA L. D.; FONSECA, CLOVIS R. S.; PELICIA, VANESSA C.; FERREIRA, FERNANDO; ABRAHAO, JONATAS S.; et al. Vaccinia virus in Feces and Urine of Wild Rodents from SAo Paulo State, Brazil. Viruses-Basel, v. 10, n. 2, FEB 2018. (13/07693-1)

PERES, MARINA G.; BACCHIEGA, THAIS S.; APPOLINARIO, CAMILA M.; VICENTE, ACACIA F.; RIBEIRO MIONI, MATEUS DE SOUZA; RIBEIRO, BRUNA L. D.; FONSECA, CLOVIS R. S.; PELICIA, VANESSA C.; FERREIRA, FERNANDO; OLIVEIRA, GRAZIELE P.; et al. Vaccinia Virus in Blood Samples of Humans, Domestic and Wild Mammals in Brazil. Viruses-Basel, v. 10, n. 1, JAN 2018. (13/07693-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SERAFIM, Marina Gea Peres. Detection of Vaccinia virus in central west region of São Paulo state. 2016. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.