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| Author(s): |
Marina Gea Peres
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Botucatu. 2016-08-30. |
| Institution: | Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu |
| Defense date: | 2016-08-01 |
| Advisor: | Jane Megid |
| Abstract | |
The present study aimed to verify positivity for Vaccinia virus in samples from domestic and wild animals, and humans from areas with and without outbreaks history, in the central west region of São Paulo State. Was randomly sampled 47 milking farms: 10 in Torre de Pedra (which registered two previous outbreak), 15 in Bofete and 22 in Anhembi (both without histories of outbreak). We assessed the presence of viral DNA in blood samples collected from cows, other domestic and wild mammals, as well as from humans and in feces and urine sample from wild rodents captured in forest areas in the 47 sampled farms. The viral DNA was detected in blood samples of four cows, one horse, three opossums (one Didelphis albiventris and two Didelphis aurita), as well as in feces and urine samples of wild rodents: six samples PCR-positive for viral DNA in feces (two Oligoryzomys flavescens, two Oligoryzomys nigripes and two Sooretamys angouya), and one for viral DNA in urine (Oligoryzomys flavescens). The sequences analysis of vgf gene show our samples clustered with Brazilian and vaccine Vaccinia virus, which allowed us to classify our samples as Vaccinia virus. Reports for the first time the detection of viral DNA in blood, feces and urine samples of healthy animals, naturally infected, and suggests that Vaccinia virus is subclinical circulating in the region sampled. Additionally suggests that wild rodents naturally infected and apparently healthy are potential infection source evidenced by elimination of Vaccinia virus in feces and urine samples. (AU) | |
| FAPESP's process: | 13/07693-1 - Phylogenetic analysis of the Vaccinia virus (VACV) circulating in wild and domestic animals of the Central West region of São Paulo State |
| Grantee: | Marina Gea Peres Serafim |
| Support Opportunities: | Scholarships in Brazil - Doctorate |