West Nile virus (WNV) is an emergent pathogen, the transmission network involves mosquitoes, birds, horses and humans. It is widely distributed in North and Central America. The recent introduction in South America has focused attention on the spread of WNV across Southern American countries, and it is coming to Brazil, where no case of the disease has not been described yet, however, there are serological reports of horses and birds positive for WNV in Brazil. Given the number of cases of emerging and re-emerging diseases reported in recent decades in the South American continent, the current cases of WNV in South America and especially the results found by our group showing the circulation of WNV in birds and equines in the Mato Grosso Pantanal region, we propose in this eco-epidemiological study, to evaluate the occurrence of WNV and other flaviviruses in their animal hosts and study their genetic variability in the Pantanal by molecular detection technique, one-step real-time RT -PCR, simultaneous tests using more specific method for the detection of WNV by TaqMan and more sensitive method for the detection of flaviviruses by SYBR Green, that may present potential competition in the establishment of WNV in Brazil, as well as serological tests. The possibility of positive samples, we will test by molecular characterization using nucleotide sequencing techniques and to focus on identifying agents never before described, we will also use method of virus isolation by cell culture. In order to establish a monitoring of WNV and other flavivirus in the Pantanal, samples of oral swabs , cloacal/anal and serum of various animals such as birds , horses and alligators will be collected, having as main goals to contribute to the strengthening of health protection services animal, increase research capacity, and finally, update and harmonize standards procedures for the prevention and control of West Nile vírus in our country. (AU)
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(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BARBOSA, CARLA M.;
DURIGON, EDISON L.;
THOMAZELLI, LUCIANO M.;
NARDI, MARCELLO SHIAVO;
DE AGUIAR, DANIEL M.;
PINHO, JOAO BATISTA;
PETRY, MARIA VIRGINIA;
NETO, ISAAC SIMAO;
RODRIGUES, ROBERTA COSTA;
DE AZEVEDO JUNIOR, SEVERINO MENDES;
GOES, LUIZ GUSTAVO B.;
DE ARAUJO, JANSEN.
Divergent coronaviruses detected in wild birds in Brazil, including a central park in SAo Paulo.
Brazilian Journal of Microbiology,
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