FLUORESCENCE SPECTROSCOPY OF TOOTH IN OVARIECTOMIZED RATS FOR OSTEOPOROSIS DIAGNOSIS

• Grant number: 13/14001-9
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: October 01, 2013
• End date: September 30, 2017
• Field of knowledge: Engineering - Biomedical Engineering - Medical Engineering
• Principal Investigator: Vanderlei Salvador Bagnato
• Grantee: Fernanda Rossi Paolillo
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 13/07276-1 - CEPOF - Optics and Photonic Research Center, AP.CEPID

Abstract

Osteoporosis is an important public health problem. The objective of this study is to investigate the fluorescence spectroscopy of tooth in ovariectomized rats for osteoporosis diagnosis. Twenty female Wistar rats will be bilaterally ovariectomized and divided into two groups: control group, without ovariectomy (CG) and group with ovariectomy (OG). Fluorescence spectroscopy of tooth and X-rays (jaw, vertebra and femur) will be performed before of ovariectomy and; 30, 60, 90 and 120 days post- ovariectomy. After euthanasia of the rats, the bones will be removed to perform a histomorphological analysis. Two-way repeated measures ANOVA and one-way ANOVA will be used. Where appropriate, post hoc comparisons will be employed (Tukey). Moreover, Pearson's product momentum correlation coefficient will be used. Future results can show calcium metabolism changes through fluorescence spectroscopy for diagnosis of osteoporosis.

Scientific publications

(21)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PAOLILLO, FERNANDA ROSSI; ROSS, ARENA; DUTRA, DANIELA BASSI; MARQUETI DURIGAN, RITA DE CASSIA; DE ARAUJO, HELOISA SELISTRE; DUTRA DE SOUZA, HUGO CELSO; PARIZOTTO, NIVALDO ANTONIO; CIPRIANO, JR., GERSON; CHIAPPA, GASPAR; BORGHI-SILVA, AUDREY. Low-level Laser Therapy Associated With High Intensity Resistance Training on Cardiac Autonomic Control of Heart Rate and Skeletal Muscle Remodeling in Wistar Rats. Lasers in Surgery and Medicine, v. 46, n. 10, p. 796-803, DEC 2014. (09/01842-0, 13/07276-1, 13/14001-9)

PAOLILLO, ALESSANDRA ROSSI; PAOLILLO, FERNANDA ROSSI; JOAO, JESSICA PATRICIA; JOAO, HERBERT ALEXANDRE; BAGNATO, VANDERLEI SALVADOR. Synergic effects of ultrasound and laser on the pain relief in women with hand osteoarthritis. Lasers in Medical Science, v. 30, n. 1, p. 279-286, JAN 2015. (13/07276-1, 13/14001-9)

PAOLILLO, FERNANDA ROSSI; BORGHI-SILVA, AUDREY; ARENA, ROSS; PARIZOTTO, NIVALDO ANTONIO; KURACHI, CRISTINA; BAGNATO, VANDERLEI SALVADOR. Effects of phototherapy plus physical training on metabolic profile and quality of life in postmenopausal women. Journal of Cosmetic and Laser Therapy, v. 19, n. 6, p. 364-372, 2017. (09/01842-0, 13/07276-1, 13/14001-9)

PAOLILLO, FERNANDA ROSSI; ROMANO, RENAN ARNON; DE MATOS, LUCIANA; SOUSA RODRIGUES, PHAMILLA GRACIELLI; PANHOCA, VITOR HUGO; MARTIN, AIRTON ABRAHAO; SILVA SOARES, LUIS EDUARDO; DE CASTRO NETO, JARBAS CAIADO; BAGNATO, VANDERLEI SALVADOR. Fluorescence spectroscopy of teeth and bones of rats to assess demineralization: In vitro, in vivo and ex vivo studies. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 165, p. 291-297, DEC 2016. (13/07276-1, 13/14001-9)

VIEIRA LEITE, DIEGO PORTES; PAOLILLO, FERNANDA ROSSI; PARMESANO, THIAGO NOGUEIRA; FONTANA, CARLA RAQUEL; BAGNATO, VANDERLEI SALVADOR. Effects of Photodynamic Therapy with Blue Light and Curcumin as Mouth Rinse for Oral Disinfection: A Randomized Controlled Trial. Photomedicine and Laser Surgery, v. 32, n. 11, p. 627-632, NOV 1 2014. (13/07276-1, 13/14001-9)

PAOLILLO, FERNANDA ROSSI; PAOLILLO, ALESSANDRA ROSSI; JOAO, JESSICA PATRICIA; FRASCA, DANIELE; DUCHENE, MARCELO; JOAO, HERBERT ALEXANDRE; BAGNATO, VANDERLEI SALVADOR. Ultrasound plus low-level laser therapy for knee osteoarthritis rehabilitation: a randomized, placebo-controlled trial. RHEUMATOLOGY INTERNATIONAL, v. 38, n. 5, p. 785-793, MAY 2018. (13/07276-1, 13/14001-9)

PAOLILLO, FERNANDA ROSSI; ROMANO, RENAN ARNON; DE MATOS, LUCIANA; MARTIN, AIRTON ABRAHAO; GONTIJO GUIMARAES, FRANCISCO EDUARDO; DE CASTRO NETO, JARBAS CAIADO; BAGNATO, VANDERLEI SALVADOR. Short-term and long-term effects of osteoporosis on incisor teeth and femoral bones evaluated by Raman spectroscopy and energy dispersive X-ray analysis in ovariectomized rats. JOURNAL OF BONE AND MINERAL METABOLISM, v. 37, n. 1, p. 18-27, JAN 2019. (13/07276-1, 13/14001-9)

PAOLILLO, FERNANDA ROSSI; LUCCAS, GIOVANA ANIELLE ALCALDE; PARIZOTTO, NIVALDO ANTONIO; PAOLILLO, ALESSANDRA ROSSI; DE CASTRO NETO, JARBAS CAIADO; BAGNATO, VANDERLEI SALVADOR. The effects of transcranial laser photobiomodulation and neuromuscular electrical stimulation in the treatment of post-stroke dysfunctions. Journal of Biophotonics, v. N/A, p. 13-pg., 2023-01-02. (13/07276-1, 13/14001-9)

DE SOUZA, VIVIANE BROCCA; FERREIRA, LAIS TATIANE; SENE-FIORESE, MARCELA; GARCIA, VANESSA; RODRIGUES, TIAGO ZUCCOLOTTO; DE AQUINO JUNIOR, ANTONIO EDUARDO; BAGNATO, VANDERLEI SALVADOR; PANHOCA, VITOR HUGO. Photobiomodulation therapy for treatment olfactory and taste dysfunction COVID-19-related: A case report. Journal of Biophotonics, v. N/A, p. 4-pg., 2022-05-17. (13/07276-1, 13/14001-9)

PANHOCA, VITOR HUGO; NOGUEIRA, MARCELO SAITO; BAGNATO, VANDERLEI SALVADOR; WONG, BJF; ILGNER, JF. Laser and vacuum therapy for treatment of facial nerve palsies. IMAGING, THERAPEUTICS, AND ADVANCED TECHNOLOGY IN HEAD AND NECK SURGERY AND OTOLARYNGOLOGY 2022, v. 11935, p. 10-pg., 2022-01-01. (13/14001-9, 13/07276-1)

PAOLILLO, FERNANDA ROSSI; TEOFILO BORGES CAMPOS, THAYS YARA; ALVAREZ, CAROLINA; SENE-FIORESE, MARCELA; BAGNATO, VANDERLEI SALVADOR; GARCIA OLIVEIRA DUARTE, ANA CLAUDIA; PARIZOTTO, NIVALDO ANTONIO. Synergic effects of ultrasound and laser therapies on mesentery for management of obesity and diabetes in rats. Journal of Biophotonics, v. 14, n. 11, AUG 2021. (13/14001-9, 13/07276-1)

PAOLILLO, FERNANDA ROSSI; LOBO DA COSTA, PAULA HENTSCHEL; BRAGA MENDES, PAULO VINICIUS; CEZAR DA CRUZ, DANIEL MARINHO; PAOLILLO, ALESSANDRA ROSSI; BAGNATO, VANDERLEI SALVADOR. Effects of the infrared laser on classical ballerinas' feet: Analysis of plantar foot and static balance. JOURNAL OF BODYWORK AND MOVEMENT THERAPIES, v. 26, p. 246-252, APR 2021. (13/07276-1, 13/14001-9)

PANHOCA, VITOR HUGO; ESTEBAN FLOREZ, FERNANDO LUIS; CORREA, THAILA QUATRINI; PAOLILLO, FERNANDA ROSSI; OLIVEIRA DE SOUZA, CLOVIS WESLEY; BAGNATO, VANDERLEI SALVADOR. Oral Decontamination of Orthodontic Patients Using Photodynamic Therapy Mediated by Blue-Light Irradiation and Curcumin Associated with Sodium Dodecyl Sulfate. Photomedicine and Laser Surgery, v. 34, n. 9, p. 411-417, SEP 2016. (13/07276-1, 13/14001-9)

PAOLILLO, FERNANDA R.; MATTOS, VICENTE S.; DE OLIVEIRA, ANDRE O.; GUIMARAES, FRANCISCO E. G.; BAGNATO, VANDERLEI S.; DE CASTRO NETO, JARBAS C.. Noninvasive assessments of skin glycated proteins by fluorescence and Raman techniques in diabetics and nondiabetics. Journal of Biophotonics, v. 12, n. 1, JAN 2019. (13/14001-9, 13/07276-1)

ADABBO, MAYNA; PAOLILLO, FERNANDA ROSSI; BOSSINI, PAULO SERGIO; RODRIGUES, NATALIA CAMARGO; BAGNATO, VANDERLEI SALVADOR; PARIZOTTO, NIVALDO ANTONIO. Effects of Low-Level Laser Therapy Applied Before Treadmill Training on Recovery of Injured Skeletal Muscle in Wistar Rats. Photomedicine and Laser Surgery, v. 34, n. 5, p. 187-193, MAY 2016. (13/07276-1, 13/14001-9)

PAOLILLO, FERNANDA ROSSI; RODRIGUES, PHAMILLA GRACIELLI SOUSA; BAGNATO, VANDERLEI SALVADOR; ALVES, FERNANDA; PIRES, LAYLA; CORAZZA, ADALBERTO VIEIRA. The effect of combined curcumin-mediated photodynamic therapy and artificial skin onStaphylococcus aureus-infected wounds in rats. Lasers in Medical Science, v. 36, n. 6, p. 1219-1226, AUG 2021. (13/07276-1, 13/14001-9)

PANHOCA, VITOR HUGO; NOGUEIRA, MARCELO SAITO; BAGNATO, VANDERLEI SALVADOR. Treatment of facial nerve palsies with laser and endermotherapy: a report of two cases. LASER PHYSICS LETTERS, v. 18, n. 1, JAN 2021. (13/14001-9, 13/07276-1)

PANHOCA, VITOR HUGO; BAGNATO, VANDERLEI SALVADOR; ALVES, NILTON; PAOLILLO, FERNANDA ROSSI; DEANA, NAIRA FIGUEIREDO. Increased Oral Health-Related Quality of Life Postsynergistic Treatment with Ultrasound and Photobiomodulation Therapy in Patients with Temporomandibular Disorders. PHOTOBIOMODULATION PHOTOMEDICINE AND LASER SURGERY, v. 37, n. 11, p. 694-699, NOV 1 2019. (13/14001-9, 13/07276-1)

PAOLILLO, FERNANDA ROSSI; MATTOS, VICENTE SILVA; BORGHI-SILVA, AUDREY; BAGNATO, VANDERLEI SALVADOR; DE CASTRO NETO, JARBAS CAIADO. Advanced Glycation Endproducts as Biomarkers for Risk of Diabetes and Cardiovascular Diseases by Skin Autofluorescence: A Noninvasive Optical Screening. PHOTOBIOMODULATION PHOTOMEDICINE AND LASER SURGERY, v. 37, n. 3, p. 168-174, MAR 2019. (13/14001-9, 13/07276-1)

PANHOCA, VITOR HUGO; DE AQUINO JUNIOR, ANTONIO EDUARDO; DE SOUZA, VIVIANE BROCCA; FERREIRA, SIMONE APARECIDA; FERREIRA, LAIS TATIANE; DE OLIVEIRA SOUZA, KARINA JULLIENNE; TAMAE, PATRICIA ERIKO; SAITO NOGUEIRA, MARCELO; BAGNATO, VANDERLEI SALVADOR. Effects of Red and Infrared Laser Therapy in Patients with Tinnitus: A Double-Blind, Clinical, Randomized Controlled Study Combining Light with Ultrasound, Drugs and Vacuum Therapy. JOURNAL OF PERSONALIZED MEDICINE, v. 13, n. 4, p. 21-pg., 2023-04-01. (13/14001-9, 13/07276-1)

PANHOCA, VITOR HUGO; FERREIRA, LAIS TATIANE; DE SOUZA, VIVIANE BROCCA; FERREIRA, SIMONE APARECIDA; SIMAO, GABRIELY; DE AQUINO JUNIOR, ANTONIO EDUARDO; BAGNATO, VANDERLEI SALVADOR; HANNA, REEM. Can photobiomodulation restore anosmia and ageusia induced by COVID-19? A pilot clinical study. Journal of Biophotonics, v. 16, n. 6, p. 13-pg., 2023-03-22. (13/14001-9, 13/07276-1)