Inhibition kinects of cellobiose enzymatic hydrolysis

• Grant number: 13/18113-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2013
• End date: July 31, 2014
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Aline Carvalho da Costa
• Grantee: Matheus Amaral Gigo
• Host Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 08/57873-8 - An integrated process for total bioethanol production and zero CO2 emission, AP.BIOEN.TEM

Abstract

The objective of this work is to study the kinetics of cellobiose enzymatic hydrolysis and assess the mechanism and the degree of inhibition of glucose on ²-glucosidase from Aspergillus niger (Novozyme 188). The experimentally measured initial velocity of the reaction will be used to determine the kinetics of inhibition by product in the following conditions: cellobiose and glucose concentrations of 1-20 g / L and 2-20 g / l, respectively. The experiments will be performed at pH 4.8, temperature 50°C and enzyme load fixed at 38.5 CBU / mL solution. The kinetic parameters (Ki, Km and Vmax) will be determined using the reciprocal Lineweaver-Burk graphics. (AU)