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Inhibition kinects of cellobiose enzymatic hydrolysis

Grant number: 13/18113-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): November 01, 2013
Effective date (End): July 31, 2014
Field of knowledge:Engineering - Chemical Engineering
Principal Investigator:Aline Carvalho da Costa
Grantee:Matheus Amaral Gigo
Home Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:08/57873-8 - An integrated process for total bioethanol production and zero CO2 emission, AP.BIOEN.TEM

Abstract

The objective of this work is to study the kinetics of cellobiose enzymatic hydrolysis and assess the mechanism and the degree of inhibition of glucose on ²-glucosidase from Aspergillus niger (Novozyme 188). The experimentally measured initial velocity of the reaction will be used to determine the kinetics of inhibition by product in the following conditions: cellobiose and glucose concentrations of 1-20 g / L and 2-20 g / l, respectively. The experiments will be performed at pH 4.8, temperature 50°C and enzyme load fixed at 38.5 CBU / mL solution. The kinetic parameters (Ki, Km and Vmax) will be determined using the reciprocal Lineweaver-Burk graphic.