Development of microsatellite markers for sharks Carcharhinus acronotus and Galeocerdo cuvier using second generation sequencing

• Grant number: 13/14555-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2013
• End date: July 31, 2015
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Fausto Foresti
• Grantee: Natália Jade Mendes
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

In the last three decades several shark species have suffered drastic population decline. The main cause is shark finning, to supply a valuable market in Asia. Among the species heavily impacted, the tiger shark (Galeocerdo cuvier) and the black snout shark (Carcharhinus acronotus), already show strong signs of population decline with both species classified as Near Threatened on the IUCN list. However studies which aim toward sustainability and proper management of fisheries remain inconsistent. It is considered that information about the population's genetic structure can contribute to the characterization of genetic diversity, geographic distribution of genetic stocks, patterns of migration, gene flow, historical events, family structures, and reproductive strategies. Data of these aspects are especially relevant to the fisheries sector, providing the basis for subsidies towards management and conservation of stocks. With the urgent need for sustainable control of fishing, this study will seek to develop microsatellite markers for characterization of polymorphisms population of C. acronotus and G. cuvier using pyrosequencing. Such markers may assist in assessments worldwide, contributing to generate substantial information on elasmobranches; in addition supporting management programs, sustainable control of fisheries, and species conservation. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MENDES, N. J.; CRUZ, V. P.; MENDONCA, FERNANDO FERNANDES; PARDO, B. G.; COELHO, R.; ASHIKAGA, F. Y.; CAMARGO, S. M.; MARTINEZ, P.; OLIVEIRA, C.; SANTOS, M. N.; et al. Microsatellite loci in the oceanic whitetip shark and cross-species amplification using pyrosequencing technology. CONSERVATION GENETICS RESOURCES, v. 7, n. 2, p. 585-589, JUN 2015. (10/51903-2, 13/14555-4, 11/23787-0)

MENDES, NATALIA J.; CRUZ, VANESSA P.; ASHIKAGA, FERNANDO Y.; CAMARGO, SAMIA M.; OLIVEIRA, CLAUDIO; PIERCY, ANDREW N.; BURGESS, GEORGE H.; COELHO, RUI; SANTOS, MIGUEL N.; MENDONCA, FERNANDO F.; et al. Microsatellite loci in the tiger shark and cross-species amplification using pyrosequencing technology. PeerJ, v. 4, AUG 30 2016. (11/23787-0, 13/14555-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MENDES, Natália Jade. Identificação de marcadores microssatélites para o tubarão Galeocerdo cuvier utilizando sequenciamento de segunda geração. 2015. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.