Influence of hemicellulose removal from sugarcane bagasse in ligin sulfonation and enzimatic hydrolysis of cellulose

• Grant number: 13/18439-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2013
• End date: October 31, 2014
• Field of knowledge: Biological Sciences - Biochemistry - Enzymology
• Principal Investigator: Adriane Maria Ferreira Milagres
• Grantee: Guilherme Galvão de Campos
• Host Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil

Abstract

The lignin and hemicellulose limit the action of cellulases in lignocelullosic materials. Hemicellulose can be removed by acids in high temperatures, though during this process the lignin attaches and redistributes itself over the cellulose hampering the cellulase accessibility. This project seeks to analyze the effects of sulfite alkaline treatment in sugarcane bagasses that has already been treated with sulfuric acid. Will be verified the rates of structural component removal of bagasse and the yields of enzymatic hydrolyze of cellulose to glucose. To reach these goals, both a bagasse "in natura", which will be used as control, and a cellulignin (bagasse treat with H2SO4 1% at 121 ºC during 10 min) will suffer the treatment with Na2SO3 and NaOH (10 e 5% w/w respectively and 5 e 2.5% w/w) and the pulps obtained are going through chemical characterization, sulfonic groups quantification and enzymatic hydrolyze. If the results are good, surpassing the treatments used nowadays, news possibilities of simplifications to this method will be tested to determinate which steps could be skipped or which reactants could be less used and keep the high results. (AU)