Research and development aiming at the integrated exploitation of sugarcane bagasse for the biotechnological production of lignocellulosic ethanol

• Grant number: 08/57926-4
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• Duration: November 01, 2009 - October 31, 2013
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Convênio/Acordo: FAPEMIG
• Principal Investigator: Silvio Silvério da Silva
• Grantee: Silvio Silvério da Silva
• Host Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil
• Pesquisadores principais: Maria das Graças de Almeida Felipe
• Associated grant(s): 12/51214-8 - Biodelignification of sugarcane bagasse for the enzymatic hydrolysis and fermentation in one pot to save water and clean energy production, AP.R
• Associated scholarship(s): 11/01226-7 - Comparative analysis of bioethanol production from sugarcane bagasse hemicellulosic hydrolysate by Pichia stipitis NRRL Y-7124 and Candida shehatae UFMG 52.2 HM yeasts aiming its application in bioprocesses with electromagnetic field, BP.DR; 10/13828-9 - Fermentative process to obtaining second GENERATIOL ethanol from sugarcane hemicellulosic hydrolysate in a basket type STR bioreactor using immobilized cells, BP.MS; 10/10446-8 - Study and optimization of sugarcane bagasse pulp digestibility using cellulolytic enzymes produced by Brazilian biodiversity isolates yeasts, BP.PD; 10/08066-2 - Study of the production of bioethanol from hemicellulose hydrolyzed of sugarcane bagasse in fluidized-bed reactor and evaluation of the environmental impact generated by residues during the process, BP.PD

Abstract

This project is a collaborative research within the BIOEN -FAPES P/FAPEMIG call between the Biotechnology Department of the Lorena School of Engineering (EEL/USP), Physics Department of the Federal University of Juiz de Fora (UFJF), Microbiology Department of the Federal University of Minas Gerais (UFMG) and Microbiology Laboratory, São Paulo State University (UNESP Rio Claro). The project aims at the fractioning of sugarcane bagasse in its main components (cellulose, hemicelluloses and lignin) for their use in the production of ethanol. The acid hydrolysis will be used to remove the hemicellulosic fraction followed by alkaline hydrolysis. The enzymatic hydrolysis of the cellulose fraction will be performed. The hydrolysates will be characterized by advanced spectroscopic techniques as Raman scattering, infra red absorption with Fourier transformation (FTIR), absorption in the near infra-red ray (NIR), thermal lens and photo-acoustic. The use of these techniques is interesting due to their nondestructive character, possibility of in situ measurements and further development of compact prototypes. In the further stage, the xylose and glucose rich hydrolysates will be properly treated by detoxification methods and used as fermentation media for the production of ethanol by xylose-fermenting yeasts and S. cerevisiae, respectively. Xylose-arabinose fermenting species isolated from the Atlantic Rain Forest, Amazon Forest and Brazilian Cerrado ecosystems will be tested. Yeasts inhabiting rotting-wood substrates will be collected and tested for xylose-arabinose fermentation. This project aims at finding new species from these Brazilian ecosystems capable to be used in industrial processes. In all the involved unitary operations in this study, conditions will be optimized by experimental design and data analysis by means of appropriate statistical methodologies. Obtained results will allow establishing advanced technologies and innovations in order to extend the national and international competitiveness of “bioenergy” to reach second generation technologies for alcohol program. It will allow the formation of teams and cooperation among the participant institutions for training and exchange of knowledge. (AU)

Scientific publications

(25)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DUSSAN, KELLY J.; SILVA, DEBORA D. V.; PEREZ, VICTOR H.; DA SILVA, SILVIO S.. Evaluation of oxygen availability on ethanol production from sugarcane bagasse hydrolysate in a batch bioreactor using two strains of xylose-fermenting yeast. RENEWABLE ENERGY, v. 87, n. 1, p. 703-710, MAR 2016. (11/01226-7, 08/57926-4)

ASCENCIO, JESUS J.; CHANDEL, ANUJ K.; PHILIPPINI, RAFAEL R.; DA SILVA, SILVIO S.. Comparative study of cellulosic sugars production from sugarcane bagasse after dilute nitric acid, dilute sodium hydroxide and sequential nitric acid-sodium hydroxide pretreatment. BIOMASS CONVERSION AND BIOREFINERY, v. 10, n. 4, NOV 2019. (08/57926-4)

CHANDEL, ANUJ K.; GONCALVES, BRUNA C. M.; STRAP, JANICE L.; DA SILVA, SILVIO S.. Biodelignification of lignocellulose substrates: An intrinsic and sustainable pretreatment strategy for clean energy production. CRITICAL REVIEWS IN BIOTECHNOLOGY, v. 35, n. 3, p. 281-293, 2015. (12/51214-8, 08/57926-4)

MILESSI, THAIS S. S.; ANTUNES, FELIPE A. F.; CHANDEL, ANUJ K.; DA SILVA, SILVIO S.. Hemicellulosic ethanol production by immobilized cells of Scheffersomyces stipitis: Effect of cell concentration and stirring. BIOENGINEERED, v. 6, n. 1, p. 26-32, JAN-FEB 2015. (08/57926-4, 10/13828-9)

DUSSAN, KELLY J.; JUSTO, OSELYS RODRIGUEZ; PEREZ, VICTOR HABER; DAVID, GERALDO F.; SILVEIRA JUNIOR, EURIPEDES GARCIA; DA SILVA, SILVIO S.. Bioethanol Production From Sugarcane Bagasse Hemicellulose Hydrolysate by Immobilized S. shehatae in a Fluidized Bed Fermenter Under Magnetic Field. BioEnergy Research, v. 12, n. 2, p. 338-346, JUN 2019. (11/01226-7, 08/57926-4)

ASCENCIO, JESUS J.; PHILIPPINI, RAFAEL R.; GOMES, FABRICIO M.; PEREIRA, FELIX M.; DA SILVA, SILVIO S.; KUMAR, VINOD; CHANDEL, ANUJ K.. Comparative Highly Efficient Production of beta-glucan by Lasiodiplodia theobromae CCT 3966 and Its Multiscale Characterization. FERMENTATION-BASEL, v. 7, n. 3, SEP 2021. (16/10636-8, 16/14852-7, 14/18714-2, 08/57926-4)

VIRGINIO DA SILVA, DEBORA DANIELLE; MACHADO, EDUARDO; DANELUSSI, OTAVIO; DOS SANTOS, MIQUEIAS GOMES; DA SILVA, SILVIO SILVERIO; DUSSAN, KELLY JOHANA. Repeated-batch fermentation of sugarcane bagasse hemicellulosic hydrolysate to ethanol using two xylose-fermenting yeasts. BIOMASS CONVERSION AND BIOREFINERY, v. N/A, p. 11-pg., 2022-01-27. (08/57926-4, 11/01226-7)

JIMENEZ, ITZCOATL M.; CHANDEL, ANUJ K.; MARCELINO, PAULO R. F.; ANJOS, VIRGILIO; COSTA, CASSIANO BATESTTIN; BELL, MARIA JOSE V.; PEREIRA, BARBARA; DA SILVA, SILVIO S.. Comparative data on effects of alkaline pretreatments and enzymatic hydrolysis on bioemulsifier production from sugarcane straw by Cutaneotrichosporon mucoides. Bioresource Technology, v. 301, APR 2020. (16/10636-8, 08/57926-4, 16/14852-7)

DUSSAN, KELLY J.; SILVA, DEBORA D. V.; PEREZ, VICTOR H.; DA SILVA, SILVIO S.. Evaluation of oxygen availability on ethanol production from sugarcane bagasse hydrolysate in a batch bioreactor using two strains of xylose-fermenting yeast. RENEWABLE ENERGY, v. 87, p. 8-pg., 2016-03-01. (08/57926-4, 11/01226-7)

CHANDEL, ANUJ K.; ANTUNES, FELIPE A. F.; SILVA, MESSIAS B.; DA SILVA, SILVIO SILVERIO. Unraveling the structure of sugarcane bagasse after soaking in concentrated aqueous ammonia (SCAA) and ethanol production by Scheffersomyces (Pichia) stipitis. BIOTECHNOLOGY FOR BIOFUELS, v. 6, JUL 15 2013. (08/57926-4)

CHANDEL, ANUJ K.; ANTUNES, FELIPE F. A.; ANJOS, VIRGILIO; BELL, MARIA J. V.; RODRIGUES, LEONARDE N.; SINGH, OM V.; ROSA, CARLOS A.; PAGNOCCA, FERNANDO C.; DA SILVA, SILVIO S.. Ultra-structural mapping of sugarcane bagasse after oxalic acid fiber expansion (OAFEX) and ethanol production by Candida shehatae and Saccharomyces cerevisiae. BIOTECHNOLOGY FOR BIOFUELS, v. 6, JAN 16 2013. (08/57926-4)

CHANDEL, ANUJ K.; CHANDRASEKHAR, G.; SILVA, MESSIAS BORGES; DA SILVA, SILVIO SILVERIO. The realm of cellulases in biorefinery development. CRITICAL REVIEWS IN BIOTECHNOLOGY, v. 32, n. 3, p. 187-202, SEP 2012. (08/57926-4)

BRANCO, RICARDO F.; DOS SANTOS, JULIO C.; SARROUH, BOUTROS F.; RIVALDI, JUAN D.; PESSOA, JR., ADALBERTO; DA SILVA, SILVIO S.. Profiles of xylose reductase, xylitol dehydrogenase and xylitol production under different oxygen transfer volumetric coefficient values. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, v. 84, n. 3, p. 326-330, 2009. (08/57926-4)

GIESE, ELLEN C.; PIEROZZI, MAURICIO; DUSSAN, KELLY J.; CHANDEL, ANUJ K.; DA SILVA, SILVIO S.. Enzymatic saccharification of acid-alkali pretreated sugarcane bagasse using commercial enzyme preparations. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, v. 88, n. 7, p. 1266-1272, JUL 2013. (08/57926-4)

CHANDEL, ANUJ K.; ANTUNES, FELIPE A. F.; ANJOS, VIRGILIO; BELL, MARIA J. V.; RODRIGUES, LEONARDE N.; POLIKARPOV, IGOR; DE AZEVEDO, EDUARDO R.; BERNARDINELLI, OIGRES D.; ROSA, CARLOS A.; PAGNOCCA, FERNANDO C.; et al. Multi-scale structural and chemical analysis of sugarcane bagasse in the process of sequential acid-base pretreatment and ethanol production by Scheffersomyces shehatae and Saccharomyces cerevisiae. BIOTECHNOLOGY FOR BIOFUELS, v. 7, APR 16 2014. (08/57926-4)

CHANDEL, ANUJ K.; DA SILVA, SILVIO SILVERIO; SINGH, OM V.. Detoxification of Lignocellulose Hydrolysates: Biochemical and Metabolic Engineering Toward White Biotechnology. BioEnergy Research, v. 6, n. 1, p. 388-401, MAR 2013. (08/57926-4)

CADETE, RAQUEL M.; MELO, MONALIZA A.; ZILLI, JERRI E.; VITAL, MARCOS J. S.; MOURO, ADRIANE; PROMPT, ALICE H.; GOMES, FATIMA C. O.; STAMBUK, BORIS U.; LACHANCE, MARC-ANDRE; ROSA, CARLOS A.. Spathaspora brasiliensis sp. nov., Spathaspora suhii sp. nov., Spathaspora roraimanensis sp. nov. and Spathaspora xylofermentans sp. nov., four novel D- xylose-fermenting yeast species from Brazilian Amazonian forest. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, v. 103, n. 2, p. 421-431, FEB 2013. (08/57926-4)

CADETE, RAQUEL M.; MELO, MONALIZA A.; DUSSAN, KELLY J.; RODRIGUES, RITA C. L. B.; SILVA, SILVIO S.; ZILLI, JERRI E.; VITAL, MARCOS J. S.; GOMES, FATIMA C. O.; LACHANCE, MARC-ANDRE; ROSA, CARLOS A.. Diversity and Physiological Characterization of D-Xylose-Fermenting Yeasts Isolated from the Brazilian Amazonian Forest. PLoS One, v. 7, n. 8, AUG 13 2012. (08/57926-4)

ASCENCIO, JESUS J.; CHANDEL, ANUJ K.; PHILIPPINI, RAFAEL R.; DA SILVA, SILVIO S.. Comparative study of cellulosic sugars production from sugarcane bagasse after dilute nitric acid, dilute sodium hydroxide and sequential nitric acid-sodium hydroxide pretreatment. BIOMASS CONVERSION AND BIOREFINERY, v. 10, n. 4, p. 813-822, DEC 2020. (08/57926-4)

GIESE, ELLEN C.; DUSSAN, KELLY J.; PIEROZZI, MAURICIO; CHANDEL, ANUJ K.; PAGNOCCA, FERNANDO C.; DA SILVA, SILVIO S.. Cellulase Production by Trichosporon laibachii. ORBITAL-THE ELECTRONIC JOURNAL OF CHEMISTRY, v. 9, n. 4, p. 271-278, JUL-SEP 2017. (08/57926-4)

VIEIRA, MATHEUS MAITAN; KADOGUCHI, ELEN; SEGATO, FERNANDO; DA SILVA, SILVIO S.; CHANDEL, ANUJ K.. Production of cellulases byAureobasidium pullulansLB83: optimization, characterization, and hydrolytic potential for the production of cellulosic sugars. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, v. 51, n. 2, AUG 2020. (14/18714-2, 16/14852-7, 16/10636-8, 08/57926-4)

PHILIPPINI, RAFAEL R.; MARTINIANO, SABRINA E.; CHANDEL, ANUJ K.; DE CARVALHO, WALTER; DA SILVA, SILVIO S.. Pretreatment of Sugarcane Bagasse from Cane Hybrids: Effects on Chemical Composition and 2G Sugars Recovery. WASTE AND BIOMASS VALORIZATION, v. 10, n. 6, p. 1561-1570, JUN 2019. (08/57926-4)

SOARES, LUMA C. S. R.; CHANDEL, ANUJ K.; PAGNOCCA, FERNANDO C.; GAIKWAD, SWAPNIL C.; RAI, MAHENDRA; DA SILVA, SILVIO S.. Screening of Yeasts for Selection of Potential Strains and Their Utilization for In Situ Microbial Detoxification (ISMD) of Sugarcane Bagasse Hemicellulosic Hydrolysate. INDIAN JOURNAL OF MICROBIOLOGY, v. 56, n. 2, p. 172-181, JUN 2016. (08/57926-4, 10/13828-9)

CHANDEL, ANUJ K.; DA SILVA, SILVIO S.; CARVALHO, WALTER; SINGH, OM V.. Sugarcane bagasse and leaves: foreseeable biomass of biofuel and bio-products. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, v. 87, n. 1, p. 11-20, JAN 2012. (08/57926-4)

ASCENCIO, JESUS J.; CHANDEL, ANUJ K.; PHILIPPINI, RAFAEL R.; DA SILVA, SILVIO S.. Comparative study of cellulosic sugars production from sugarcane bagasse after dilute nitric acid, dilute sodium hydroxide and sequential nitric acid-sodium hydroxide pretreatment. BIOMASS CONVERSION AND BIOREFINERY, v. 10, n. 4, p. 10-pg., 2019-11-27. (08/57926-4)