Repeated-batch fermentation of sugarcane bagasse hemicellulosic hydrolysate to ethanol using two xylose-fermenting yeasts

Author(s)

Virginio da Silva, Debora Danielle ; Machado, Eduardo ; Danelussi, Otavio ; dos Santos, Miqueias Gomes ; da Silva, Silvio Silverio ; Dussan, Kelly Johana

• Document type: Journal article
• Source: BIOMASS CONVERSION AND BIOREFINERY; v. N/A, p. 11-pg., 2022-01-27.

Abstract

In this study, a repeated-batch fermentation of hemicellulosic sugarcane bagasse hydrolysate was investigated to produce bioethanol using two xylose-fermenting yeasts, Scheffersomyces stipitis and Scheffersomyces shehatae. First, diluted-acid pretreatment of sugarcane bagasse at 150 mg H2SO4/g dry bagasse and 127 degrees C for 10 min, followed by concentration (3.5-fold), and detoxification process led to a hemicellulosic hydrolysate containing 48.97 g L-1 xylose. The hemicellulosic hydrolysate was fermented using repeated-batch operation and studying the performance of S. stipitis and S. shehatae yeasts. The results showed that fermentative parameters through the repeated-batch were significantly increased, and the ability of yeasts was maintained during the two cycles (a total of third rounds). Furthermore, S. shehatae was 85% more efficient in xylose to ethanol conversion than S. stipitis, producing 21.5 g L-1 ethanol that corresponds to Y-P/S = 0.436 g g(-1) and Q(P) = 0.241 g L-1 h(-1). In general, the use of sugarcane bagasse hemicellulosic fraction has been proved an excellent alternative to boost bioethanol production together the use of cellulose fraction, to replace fossil fuels. This work showed that the repeated-batch operation and S. shehatae yeast are possible combinations for industrial ethanol production from lignocellulosic hydrolysate. (AU)

• FAPESP's process: 08/57926-4 - Research and development aiming at the integrated exploitation of sugarcane bagasse for the biotechnological production of lignocellulosic ethanol
• Grantee: Silvio Silvério da Silva
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 11/01226-7 - Comparative analysis of bioethanol production from sugarcane bagasse hemicellulosic hydrolysate by Pichia stipitis NRRL Y-7124 and Candida shehatae UFMG 52.2 HM yeasts aiming its application in bioprocesses with electromagnetic field
• Grantee: Kelly Johana Dussán Medina
• Support Opportunities: Scholarships in Brazil - Doctorate