Effect of low-temperature plasma on pathogenic oral biofilms and in vitro reconstituted oral epithelium

• Grant number: 13/21388-7
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: February 17, 2014
• End date: May 30, 2014
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Carlos Eduardo Vergani
• Grantee: Juliana Aparecida Delben
• Supervisor: Simone Duarte
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: New York University, United States
• Associated to the scholarship: 12/20699-6 - Efficacy and toxicity of low-temperature plasma on pathogenic biofilms developed in in vitro reconstituted human epithelium, BP.PD

Abstract

Considering the ability of low-temperature plasma (LTP) to disrupt the biofilm matrix and rupture cell structure, it can be an efficient tool against virulent oral biofilms. However, it is also important that LTP does not cause damage to oral tissue. So, this study will evaluate (1) the antimicrobial effect of LTP on single- and multi-species biofilms of C. albicans, methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) as well as (2) the toxicity of LTP on non-infected in vitro reconstituted oral epithelium and (3) in vitro reconstituted oral epithelium infected with the same single- and multi-specie biofilms. Standardized cell suspension of each microorganism will be prepared for culture of biofilm on acrylic resin discs at 37°C for 48 hours. Then, the biofilms will be submitted to LTP treatment at standardized plasma tip-to-sample distance. The samples will be analyzed through counting of viable colonies, confocal laser scanning microscopy (CLSM) and environmental scanning electron microscopy. Non-infected in vitro reconstituted oral epithelium will be submitted to similar LTP treatment and analyzed through histology, cytotoxicity analysis and CLSM. Additional samples of in vitro reconstituted oral epithelium will be infected with standardized cell suspensions of C. albicans, MSSA and MRSA for culture of single- and multi-specie biofilms at 37°C for 24 hours and submitted to similar LTP treatment. These samples will be also analyzed through histology, cytotoxicity analysis and CLSM. Quantitative data will be evaluated using appropriate statistical tests while images will be evaluated through qualitative observational methods. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DELBEN, JULIANA APARECIDA; ZAGO, CHAIENE EVELIN; TYHOVYCH, NATALIA; DUARTE, SIMONE; VERGANI, CARLOS EDUARDO. Effect of Atmospheric-Pressure Cold Plasma on Pathogenic Oral Biofilms and In Vitro Reconstituted Oral Epithelium. PLoS One, v. 11, n. 5, MAY 25 2016. (12/20699-6, 13/21388-7)