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Use of low temperature atmospheric pressure plasma in dentistry: from laboratory bench to clinics

Grant number: 19/05856-7
Support type:Research Projects - Thematic Grants
Duration: February 01, 2020 - January 31, 2025
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal researcher:Cristiane Yumi Koga Ito
Grantee:Cristiane Yumi Koga Ito
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
Pesquisadores principais:
Konstantin Georgiev Kostov ; Rodrigo Savio Pessoa
Assoc. researchers:Gabriela de Morais Gouvêa Lima ; Helena Maria Marcolla Araujo ; Marcelo Vidigal Caliari ; Marcia Hiromi Tanaka ; Marcia Pinto Alves Mayer ; Paulo Francisco Guerreiro Cardoso ; Simone Duarte ; Thalita Mayumi Castaldelli Nishime
Associated scholarship(s):21/05351-2 - Study of properties of cold atmospheric plasma jet designed for inactivation of different microorganisms and healing of living tissues, BP.TT
21/03620-6 - Application and characterization of a gliding arc plasma jet in the activation of liquids for applications related to Dentistry, BP.IC
21/02680-5 - Study of cold plasma jet operating in atmospheric pressure for biomedical applications, BP.DD
+ associated scholarships 21/00046-7 - Association of L-ascorbic acid and cold atmospheric pressure plasma in the treatment of oral candidiasis: in vitro and in vivo evaluation, BP.PD
19/25652-7 - Low-temperature plasma under atmospheric pressure as an adjuvant for the treatment of Oral Mucositis, BP.DR
20/10450-7 - Exploring the potential of plasma activated liquid (PAL): characterization of plasma and application of PAL in endodontic treatment, BP.PD
20/09481-5 - Applications of non-thermal atmospheric plasmas in Dentistry: from the bench to the clinic, BP.PD - associated scholarships


This proposal aims to investigate the use of low temperature atmospheric pressure plasma (LTAPP) in Dentistry. The general aim is to develop protocols for the application of plasma and plasma activated liquid in vivo and ex vivo for diseases of dental interest and a portable equipment for clinical use. The specific aims are to develop effective protocols to the following relevant pathological conditions in Dentistry: oral candidosis, periodontitis, dental caries, endodontic infection and oral mucositis. Protocols of direct plasma application or plasma treated liquid (PTL, indirect application) will be tested. In case of infectious diseases, the effective parameters of LTAPP against each specific pathogenic biofilm will be standardized in vitro. After, these parameters will be evaluated for cyto and genotoxicity, using the most suitable cell line for each case. The effective (maximum antimicrobial effect) and safe (lowest toxicity) will be applied in vivo or ex vivo for validation. In relation to mucositis, the protocols of direct or indirect application will be evaluated in rats treated with 5-fluorouracyl, followed by histologic and immunohistochemical analyses. Systemic toxicity of PTL will be evaluated using Drosophila melanogaster model. The plasma device to be used in this study was developed and validated by the team of this project, with the support of several publications. For this reason, it is also aim of this project to transpose the current equipment to a small and portable prototype, maintaining its potentialities. (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CHIAPPIM, WILLIAM; SAMPAIO, ALINE; MIRANDA, FELIPE; PETRACONI, GILBERTO; DA SILVA SOBRINHO, ARGEMIRO; CARDOSO, PAULO; KOSTOV, KONSTANTIN; KOGA-ITO, CRISTIANE; PESSOA, RODRIGO. Nebulized plasma-activated water has an effective antimicrobial effect on medically relevant microbial species and maintains its physicochemical properties in tube lengths from 0.1 up to 1.0 m. Plasma Processes and Polymers, v. 18, n. 11 AUG 2021. Web of Science Citations: 1.
NASCIMENTO, FELLYPE; PETROSKI, KLEBER; KOSTOV, KONSTANTIN. Effects of O-2 Addition on the Discharge Parameters and Production of Reactive Species of a Transferred Atmospheric Pressure Plasma Jet. APPLIED SCIENCES-BASEL, v. 11, n. 14 JUL 2021. Web of Science Citations: 0.
CHIAPPIM, WILLIAM; SAMPAIO, ALINE DA GRACA; MIRANDA, FELIPE; FRAGA, MARIANA; PETRACONI, GILBERTO; DA SILVA SOBRINHO, ARGEMIRO; KOSTOV, KONSTANTIN; KOGA-ITO, CRISTIANE; PESSOA, RODRIGO. Antimicrobial Effect of Plasma-Activated Tap Water on Staphylococcus aureus, Escherichia coli, and Candida albicans. WATER, v. 13, n. 11 JUN 2021. Web of Science Citations: 0.
BORGES, ALINE C.; KOSTOV, KONSTANTIN G.; PESSOA, RODRIGO S.; DE ABREU, GERALDO M. A.; LIMA, GABRIELA DE M. G.; FIGUEIRA, LEANDRO W.; KOGA-ITO, CRISTIANE Y. Applications of Cold Atmospheric Pressure Plasma in Dentistry. APPLIED SCIENCES-BASEL, v. 11, n. 5 MAR 2021. Web of Science Citations: 0.
FIGUEIRA, LEANDRO W.; PANARIELLO, BEATRIZ H. D.; KOGA-ITO, CRISTIANE Y.; DUARTE, SIMONE. Low-Temperature Plasma as an Approach for Inhibiting a Multi-Species Cariogenic Biofilm. APPLIED SCIENCES-BASEL, v. 11, n. 2 JAN 2021. Web of Science Citations: 0.

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