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Low-temperature plasma under atmospheric pressure as an adjuvant for the treatment of Oral Mucositis

Grant number: 19/25652-7
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2021
Effective date (End): November 30, 2023
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Cristiane Yumi Koga Ito
Grantee:Aline da Graça Sampaio
Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
Associated research grant:19/05856-7 - Use of low temperature atmospheric pressure plasma in dentistry: from laboratory bench to clinics, AP.TEM
Associated scholarship(s):22/04723-6 - Application of low temperature cold plasma in the treatment of mucositis using an organotypic 3D model, BE.EP.DR


Oral Mucositis is a clinical condition that frequently affects patients under antineoplasic therapy for head and neck cancer. The disease is correlated with oral mucosa ulcerations and intense pain, odynophagia, increase in the risk for infections, use of antibiotics and period of hospitalization. The correlation among Oral Mucositis, fungemia and the potential of systemic fungal dissemination was recently described. Despite of the impact of this clinical condition on the lifetime and quality of life in cancer patients, there is no consensus on the prophylaxis and therapeutic protocol. Low Temperature Atmospheric Pressure Plasma (LTAPP) has antimicrobial, anti-inflammatory and tissue repair effects. These features suggest that it might be a promising tool in the treatment of Oral Mucositis. The aim of this project is to evaluate the application of LAPTT as adjuvant in the treatment of Oral Mucositis. A total of 120 rats (Rattus norvegicus) with 90 to 100 days of age will be included in the study. Oral Mucositis will be induced by systemic adminstration of cisplatin and 5-fluorouracyl associated with topic application of 50% acetic acid. The animals will be randomly divided into 4 experimental groups (n=30): i) Oral Mucositis; ii) Oral Mucositis+LTAPP; iii) Oral Mucositis infected with C. albicans and iv) Oral Mucositis infected with C. albicans+LTAPP. Treatments with LTAPP will be performed in 2 sessions after the diagnosis of Oral Mucositis. During the experimental period, the lesions will be photographed and their areas will be measured. The local microcirculation will be assessed by images of videomicroscopy analyzed by AVA 4.0 software (sidestream dark field imaging). One, 3 and 5 days after the last treatment, group of 10 animals will be euthanized. Specimens will be submitted to histologic and immunohistochemical analyses (Ki-67 and anti-VEGF), RT PCR (expression of TNF-alpha, IL-1², IL-10 and Nrf2, HO-1, Nqo1) and cell oxidation evaluation. The occurrence of fungal systemic dissemination in group of Mucositis infected with C. albicans will be assessed by fungal isolation from total blood and organs macerated. The results will be analyzed statistically by suitable tests to be selected according to data distribution and the evaluation. The level of significance will be set at 5%. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CHIAPPIM, WILLIAM; SAMPAIO, ALINE DA GRACA; MIRANDA, FELIPE; FRAGA, MARIANA; PETRACONI, GILBERTO; DA SILVA SOBRINHO, ARGEMIRO; KOSTOV, KONSTANTIN; KOGA-ITO, CRISTIANE; PESSOA, RODRIGO. ntimicrobial Effect of Plasma-Activated Tap Water on Staphylococcus aureus, Escherichia coli, and Candida albican. WATER, v. 13, n. 11, . (19/25652-7, 19/05856-7, 18/01265-1, 20/10450-7)
CHIAPPIM, WILLIAM; SAMPAIO, ALINE; MIRANDA, FELIPE; PETRACONI, GILBERTO; DA SILVA SOBRINHO, ARGEMIRO; CARDOSO, PAULO; KOSTOV, KONSTANTIN; KOGA-ITO, CRISTIANE; PESSOA, RODRIGO. Nebulized plasma-activated water has an effective antimicrobial effect on medically relevant microbial species and maintains its physicochemical properties in tube lengths from 0.1 up to 1.0 m. Plasma Processes and Polymers, v. 18, n. 11, . (19/25652-7, 19/05856-7, 20/10450-7)

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