Dermal pharmacokinetics of topical formulations determined by in vivo microdialysis

• Grant number: 13/22817-9
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: May 01, 2014
• End date: April 30, 2015
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Osvaldo de Freitas
• Grantee: Frederico Severino Martins
• Supervisor: Hartmut Derendorf
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: University of Florida, Gainesville (UF), United States
• Associated to the scholarship: 12/15979-0 - Systematic study of the melanogenic action of the extract of Brosimum gaudichaudii Trécul, BP.DR

Abstract

Microdialysis is a sampling technique to sample substances in the extracellular space and your principle can be compared to an artificial blood vessel. The probe is permeable to water and small molecules, is continuously perfused with physiological solution and the drug cross the membrane probe.The purpose this work is investigate the potential of improving dermal psoralen and bergapten delivery by formulation in microemulsion or hydrogel vehicles and to establish a reliable pharmacokinetic model to analyze cutaneous microdialysis data. After a topical application (dermal rats) of microemulsions or hydrogel, unbound cutaneous concentrations of psoralen and bergapten will be determined by in vivo microdialysis. (AU)