pH AND TEMPERATURE SENSITIVE DRUG NANOCARRIERS FOR SKIN APPLICATIONS

• Grant number: 17/24402-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): July 01, 2018
• Effective date (End): July 01, 2021
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Daniele Ribeiro de Araujo
• Grantee: Estefânia Vangelie Ramos Campos
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Associated research grant: 14/14457-5 - Lipid-based nanocarriers (SLN/NLC and remote-loading liposomes) used to improve the upload and potency of local anesthetics, AP.TEM
• Associated scholarship(s): 19/05100-0 - Evaluation of the biological activity of topical formulations of budesonide in inflammatory skin models, BE.EP.PD

Abstract

Smart drug carrier systems have the objective of modifying the limiting physicochemical properties of the drugs, enhancing their therapeutic effect, controlling their absorption and distribution in the tissues and reducing the toxicological effects. Nanoencapsulation of bioactive molecules and their subsequent mobilization in semi-solid topical formulations is a technologically viable alternative to modulate the permeation of these drugs through the skin. Within this context, the main objective of this project is to develop carrier systems that are pH and / or temperature sensitive in order to improve the availability and therapeutic efficiency of formulations for local drug delivery (topical administration), using benzocaine as a model drug. In the first stage of its execution will be prepared and characterized colloidal dispersions (nanoparticles and polymeric micelles) fluorescently labeled or not and will be evaluated for physical-chemical stability, not only along the time of storage, but also at different pH and temperatures. Next, the incorporation of these nanoformulations into hydrogel systems will be studied and their in vitro release and permeation kinetics will be carefully evaluated. In vitro cell cytotoxicity and uptake assays will be performed to evaluate the toxicity of these systems as well as the distribution of these nanoparticles. In this way, the development of carrier systems that are capable of improving the bioavailability and pharmacokinetics of drugs through the topical route is expected with the execution of this project.

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARTOLUCCI, CECILIA; ANTONACCI, AMINA; ARDUINI, FABIANA; MOSCONE, DANILA; FRACETO, LEONARDO; CAMPOS, ESTEFANIA; ATTAALLAH, RAOUIA; AMINE, AZIZ; ZANARDI, CHIARA; CUBILLANA-AGUILERA, LAURA M.; et al. Green nanomaterials fostering agrifood sustainability. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 125, APR 2020. (19/05100-0, 17/21004-5, 17/24402-1)

CAMPOS, ESTEFANIA V. R.; PROENCA, PATRICIA L. F.; DA COSTA, TAIS GERMANO; DE LIMA, RENATA; HEDTRICH, SARAH; FRACETO, LEONARDO FERNANDES; DE ARAUJO, DANIELE RIBEIRO. Hydrogels Containing Budesonide-Loaded Nanoparticles to Facilitate Percutaneous Absorption for Atopic Dermatitis Treatment Applications. ACS APPLIED POLYMER MATERIALS, v. 3, n. 9, p. 4436-4449, SEP 9 2021. (19/05100-0, 19/20303-4, 17/24402-1)

GOMES, SUSANA I. L.; CAMPOS, ESTEFANIA V. R.; FRACETO, LEONARDO F.; GRILLO, RENATO; SCOTT-FORDSMAND, JANECK J.; AMORIM, MONICA J. B.. High-throughput transcriptomics reveals the mechanisms of nanopesticides - nanoformulation, commercial formulation, active ingredient - finding safe and sustainable-by-design (SSbD) options for the environment. ENVIRONMENTAL SCIENCE-NANO, v. 9, n. 6, p. 13-pg., 2022-05-04. (17/21004-5, 17/24402-1)

CAMPOS, ESTEFANIA V. R.; PROENCA, PATRICIA L. F.; DA COSTA, TAIS G.; DE LIMA, RENATA; FRACETO, LEONARDO F.; DE ARAUJO, DANIELE R.. Using Chitosan-Coated Polymeric Nanoparticles-Thermosensitive Hydrogels in association with Limonene as Skin Drug Delivery Strategy. BIOMED RESEARCH INTERNATIONAL, v. 2022, p. 18-pg., 2022-04-07. (19/05100-0, 17/24402-1, 19/20303-4)

CAMPOS, ESTEFANIA VANGELIE RAMOS; DE OLIVEIRA, JHONES LUIZ; ABRANTES, DANIELE CARVALHO; ROGERIO, CAROLINA BARBARA; BUENO, CAROLINA; MIRANDA, VANESSA REGINA; MONTEIRO, RENATA APARECIDA; FRACETO, LEONARDO FERNANDES. Recent Developments in Nanotechnology for Detection and Control of Aedes aegypti-Borne Diseases. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v. 8, FEB 20 2020. (17/24402-1, 18/02404-5)

BABITY, SAMUEL; COUTURE, FREDERIC; CAMPOS, ESTEFANIA V. R.; HEDTRICH, SARAH; HAGEN, RAPHAEL; FEHR, DANIEL; BONMARIN, MATHIAS; BRAMBILLA, DAVIDE. A Naked Eye-Invisible Ratiometric Fluorescent Microneedle Tattoo for Real-Time Monitoring of Inflammatory Skin Conditions. ADVANCED HEALTHCARE MATERIALS, DEC 2021. (17/24402-1, 19/05100-0)

RAMOS CAMPOS, ESTEFANIA VANGELIE; PROENCA, PATRICIA LUIZA DE FREITAS; DORETTO-SILVA, LORENA; ANDRADE-OLIVEIRA, VINICIUS; FRACETO, LEONARDO FERNANDES; DE ARAUJO, DANIELE RIBEIRO. Trends in nanoformulations for atopic dermatitis treatment. Expert Opinion on Drug Delivery, v. 17, n. 11, AUG 2020. (19/20303-4, 17/24402-1, 19/05100-0)

ABRANTES, DANIELE CARVALHO; ROGERIO, CAROLINA BARBARA; DE OLIVEIRA, JHONES L.; CAMPOS, ESTEFANIA V. R.; DE ARAUJO, DANIELE RIBEIRO; PAMPANA, LAURINDO CESAR; DUARTE, MARCELO JOSE; VALADARES, GEORGIO FREESZ; FRACETO, LEONARDO FERNANDES. Development of a Mosquito Repellent Formulation Based on Nanostructured Lipid Carriers. FRONTIERS IN PHARMACOLOGY, v. 12, OCT 11 2021. (18/02404-5, 17/24402-1, 18/14734-0, 20/12779-6)

LUIS, ANGELICA I. S.; CAMPOS, ESTEFANIA V. R.; OLIVEIRA, JHONES L.; VALLIM, JOSE HENRIQUE; PROENCA, PATRICIA L. F.; CASTANHA, RODRIGO F.; DE CASTRO, VERA L. S. S.; FRACETO, LEONARDO F.. Ecotoxicity evaluation of polymeric nanoparticles loaded with ascorbic acid for fish nutrition in aquaculture. JOURNAL OF NANOBIOTECHNOLOGY, v. 19, n. 1, MAY 31 2021. (18/21142-1, 17/21004-5, 17/24402-1)

ABRANTES, DANIELE CARVALHO; ROGERIO, CAROLINA BARBARA; CAMPOS, ESTEFANIA VANGELIE RAMOS; GERMANO-COSTA, TAIS; VIGATO, ARYANE ALVES; MACHADO, IAN POMPERMEYER; SEPULVEDA, ANDERSON FERREIRA; LIMA, RENATA; DE ARAUJO, DANIELE RIBEIRO; FRACETO, LEONARDO FERNANDES. Repellent active ingredients encapsulated in polymeric nanoparticles: potential alternative formulations to control arboviruses. JOURNAL OF NANOBIOTECHNOLOGY, v. 20, n. 1, p. 16-pg., 2022-12-10. (17/24402-1, 19/14773-8, 18/02404-5, 18/14734-0)

CAMPOS, ESTEFANIA V. R.; PEREIRA, ANDERSON E. S.; DE OLIVEIRA, JHONES LUIZ; CARVALHO, LUCAS BRAGANCA; GUILGER-CASAGRANDE, MARIANA; DE LIMA, RENATA; FRACETO, LEONARDO FERNANDES. How can nanotechnology help to combat COVID-19? Opportunities and urgent need. JOURNAL OF NANOBIOTECHNOLOGY, v. 18, n. 1, SEP 5 2020. (17/21004-5, 19/05100-0, 18/21142-1, 18/23608-8, 20/05816-2, 17/24402-1)