|Support type:||Scholarships in Brazil - Scientific Initiation|
|Effective date (Start):||November 01, 2013|
|Effective date (End):||October 31, 2014|
|Field of knowledge:||Biological Sciences - Genetics - Human and Medical Genetics|
|Principal Investigator:||Lucas Trevizani Rasmussen|
|Grantee:||Luanna Munhoz Zabaglia|
|Home Institution:||Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Sagrado Coração (USC). Bauru , SP, Brazil|
Tumor necrosis factor (TNF), is a proinflammatory cytokine and some studies demonstrate that TNF-alpha acts as a potent inhibitor of gastric secretion after Helicobacter pylori infection, contributing to the development of gastric diseases, and acting as the most powerful mediator of acute inflammatory response against Gram-negative bacterium. In additional, the Helicobacter pylori (H. pylori) a Gram-negative bacterium, which persistently colonizes gastric mucosa of humans and plays an important role in the gastrointestinal diseases and appears to induce the production of TNF-alpha, contributing to the development of peptic diseases. In view of the involvement of TNF-alpha and the presence of H. pylori in development of gastric diseases, this project aims characterize the promoter region polymorphism -308 (rs1800629) of TNF by PCR-RFLP and analysis the gene expression of TNF-alpha gene in 125 samples from gastric biopsies of adults patients with peptic symptoms and / or gastric cancer, in order to correlate genotypes and the genes expression of TNF-alpha with the presence of H. pylori and some virulence markers previous detected. The development of this approach may open new perspectives for the characterization of factors involved in peptic diseases, gene regulation, as well as to elucidate the interaction among genes involved in gastric diseases, improving the understanding of the pathophysiological mechanisms of these diseases.