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Genotyping and Gene Expression Analysis of TNF-Alpha in samples from patients with gastric disease: A Possible relationship with Helicobacter pylori.

Grant number: 13/21224-4
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): November 01, 2013
Effective date (End): October 31, 2014
Field of knowledge:Biological Sciences - Genetics - Human and Medical Genetics
Principal Investigator:Lucas Trevizani Rasmussen
Grantee:Luanna Munhoz Zabaglia
Home Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Sagrado Coração (USC). Bauru , SP, Brazil

Abstract

Tumor necrosis factor (TNF), is a proinflammatory cytokine and some studies demonstrate that TNF-alpha acts as a potent inhibitor of gastric secretion after Helicobacter pylori infection, contributing to the development of gastric diseases, and acting as the most powerful mediator of acute inflammatory response against Gram-negative bacterium. In additional, the Helicobacter pylori (H. pylori) a Gram-negative bacterium, which persistently colonizes gastric mucosa of humans and plays an important role in the gastrointestinal diseases and appears to induce the production of TNF-alpha, contributing to the development of peptic diseases. In view of the involvement of TNF-alpha and the presence of H. pylori in development of gastric diseases, this project aims characterize the promoter region polymorphism -308 (rs1800629) of TNF by PCR-RFLP and analysis the gene expression of TNF-alpha gene in 125 samples from gastric biopsies of adults patients with peptic symptoms and / or gastric cancer, in order to correlate genotypes and the genes expression of TNF-alpha with the presence of H. pylori and some virulence markers previous detected. The development of this approach may open new perspectives for the characterization of factors involved in peptic diseases, gene regulation, as well as to elucidate the interaction among genes involved in gastric diseases, improving the understanding of the pathophysiological mechanisms of these diseases.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ZABAGLIA, LUANNA MUNHOZ; FERRAZ, MARIANE AVANTE; PEREIRA, WEENDELLY NAYARA; ORCINI, WILSON APARECIDO; DE LABIO, ROGER WILLIAN; NETO, AGOSTINHO CALEMAN; WISNIESKI, FERNANDA; DE OLIVEIRA, JULIANA GARCIA; CARDOSO SMITH, MARILIA DE ARRUDA; MARQUES PAYAO, SPENCER LUIZ; RASMUSSEN, LUCAS TREVIZANI. Lack of association among TNF-alpha gene expression,-308 polymorphism (G > A) and virulence markers of Helicobacter pylori. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 21, DEC 30 2015. Web of Science Citations: 4.
LUANNA MUNHOZ ZABAGLIA; MARIANE AVANTE FERRAZ; WEENDELLY NAYARA PEREIRA; WILSON APARECIDO ORCINI; ROGER WILLIAN DE LABIO; AGOSTINHO CALEMAN NETO; FERNANDA WISNIESKI; JULIANA GARCIA DE OLIVEIRA; MARILIA DE ARRUDA CARDOSO SMITH; SPENCER LUIZ MARQUES PAYÃO; LUCAS TREVIZANI RASMUSSEN. Lack of association among TNF-α gene expression, -308 polymorphism (G > A) and virulence markers of Helicobacter pylori. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 21, p. -, 2015.

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