Detection of Virulence Marker of H. pylori, oipA gene, Polymorphism Characterization + 144 and analysis of gene expression of Interleukin 2 in inflamatory gastric disease and gastric cancer

• Grant number: 15/15253-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): October 01, 2015
• Effective date (End): March 31, 2017
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal Investigator: Lucas Trevizani Rasmussen
• Grantee: Mayara Luciana Sallas
• Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Sagrado Coração (USC). Bauru , SP, Brazil

Abstract

Helicobacter pylori is a gram-negative bacterium that infects about 50% of world population, and is associated with diseases such as gastritis, ulcers and gastric carcinomas. Genetic characteristics of the bacteria differ in virulence factors, which when present are associated with a higher degree of pathogenicity. Combined with bacterial virulence markers, host genetic factors can also contribute to the development of gastric diseases, including cytokines are associated with severe inflammatory response caused by bacteria. The oipA gene, an important virulence marker seems to be associated with development of gastric disorders when in its functional form, as well as Interleukin 2 which presents the polymorphism +114, which is associated with gastric pathology been studied. Therefore this project aims to: Detect pathogenicity marker of H. pylori gene oipA; evaluating the allele and genotype frequencies of the polymorphism +114 to analyze the expression of the IL-2 gene by PCR - RFLP and PCR Real Time, respectively, in order to associate genotypes found the expression of IL- 2 and correlates them the presence of H. pylori and the oipA gene. We will be analyzed 30 samples from individuals diagnosed with gastric cancer and 120 samples from dyspeptic patients totaling 300 samples, 150 of DNA and RNA 150. The development of this approach may open new perspectives for the characterization of factors involved in peptic disease and gene regulation, aimed at a better understanding of the pathophysiological mechanisms of these diseases.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SALLAS, MAYARA LUCIANA; DOS SANTOS, MONICA PEZENATTO; ORCINI, WILSON APARECIDO; DAVID, ERICA BOARATO; PERUQUETTI, RITA LUIZA; MARQUES PAYAO, SPENCER LUIZ; RASMUSSEN, LUCAS TREVIZANI. Status (on/off) of oipA gene: their associations with gastritis and gastric cancer and geographic origins. Archives of Microbiology, v. 201, n. 1, p. 93-97, JAN 2019. (15/15253-7, 15/11371-5)

SALLAS, MAYARA LUCIANA; MELCHIADES, JESSICA LIMA; ZABAGLIA, LUANNA MUNHOZ; DO PRADO MORENO, JULIANA RIBEIRO; ORCINI, WILSON APARECIDO; CHEN, ELIZABETH SUCHI; CARDOSO SMITH, MARILIA DE ARRUDA; MARQUES PAYAO, SPENCER LUIZ; RASMUSSEN, LUCAS TREVIZANI. Prevalence of Helicobacter pylori vacA, cagA, dupA and oipA Genotypes in Patients with Gastric Disease. ADVANCES IN MICROBIOLOGY, v. 7, n. 1, p. 1-9, JAN 2017. (15/11371-5, 15/15253-7)