Profile expression of MMP-2, MMP-9 and its specific inhibitors in UPJ children’s

• Grant number: 13/17174-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2013
• End date: November 30, 2014
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Carlo Camargo Passerotti
• Grantee: Guilherme Rissato Sabioni
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil

Abstract

The Stenosis Ureteropelvic Junction (UPJ) is characterized by the obstruction of urine flow from the renal pelvis to the proximal ureter. This pathological condition more prevalent in children is the leading cause of onefrose. The obstruction can be extrinsic (constriction by anomalous vessels) and/or intrinsic. Although abnormalities have been evident in the pattern of innervation in the smooth muscle layer of the composition and collagen mechanisms essential to the understanding of the intrinsic obstruction are controversial. Matrix metalloproteinases (MMPs), secreted by mesenchymal tissue, are proteolytic enzymes important physiological processes such as tissue reparamento, morphogenesis, and angiogenesis because they degrade extracellular matrix components including collagen, however, dysregulation of these enzymes is neurotoxic (degradation of collagen type I, which protects neurons from cell death culture cytotoxic) and is found in UPJ stenosis. Therefore, the knowledge of the expression of MMPs and their inhibitors is important for specific learning of the pathophysiology of this disease. So we decided to evaluate the expression pattern of two major enzymes in this family that are MMP-2 and MMP-9 and their specific inhibitors (TIMP-1, TIMP-2, and RECK) in tissue samples JUP, and correlate the expression with the degree of hydronephrosis and renal function. (AU)