Finasteride effects on normal and tumoral prostatic cells: micro-RNA expression, cellular viability and cellular invasivity analysis

• Grant number: 10/16671-3
• Support Opportunities: Regular Research Grants
• Duration: December 01, 2010 - November 30, 2012
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Sérgio Luis Felisbino
• Grantee: Sérgio Luis Felisbino
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Andrei Moroz ; Flávia Karina Delella

Abstract

Prostate cancer (PCa) is the most diagnosed cancer and the second cause of death by cancer among American and Western European men. In Brazil, the number of new PCa cases estimated to 2010 year is about 52.350. The PCa development and progression is a slow process and involve genetic and epigenetic alterations that unbalance the molecular mechanism responsible by the control of cellular proliferation, differentiation and apoptosis. Thus, prevention therapies against PCa have been proposed. Recently, Finasteride (Proscar®), an inhibitor used in the benign prostatic hyperplasia treatment, was approved by American Society of Urology as chemopreventive treatment for high risk PCa individuals. Nevertheless, others Urology Societies alerted the need of more studies for a safety use of Finasteride in prolonged chemoprevention protocols. Recent discoveries indicate that human tumors present epigenetic alterations, like dysregulated micro-RNAs expression (molecules that are considerate new oncogenes or tumoral suppressors) and matrix metalloproteinases (MMPs) dysregulated expression (enzymes related with progression, invasion and metastasis of PCa. Bearing in mind the importance of PCa prevention and the questions about Finasteride effects on normal and tumoral prostate cells, the aim of this project is investigate Finasteride effects on some oncogenic and tumoral suppresors micro-RNAs and on protein expression and enzymatic activity of MMPs 2, and 9 and their tissue inhibitors TIMP1 and 2 in normal and tumoral prostatic epithelial cells in vitro. This project is directly related with an already proved Post-doctoral project (grant FAPESP number 2010-05042-5). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MOROZ, ANDREI; DELELLA, FLAVIA K.; LACORTE, LIVIA M.; DEFFUNE, ELENICE; FELISBINO, SERGIO L.. Fibronectin induces MMP2 expression in human prostate cancer cells. Biochemical and Biophysical Research Communications, v. 430, n. 4, p. 1319-1321, JAN 25 2013. (10/16671-3)

MOROZ, ANDREI; DELELLA, FLAVIA K.; ALMEIDA, RODRIGO; LACORTE, LIVIA MARIA; FAVARO, WAGNER JOSE; DEFFUNE, ELENICE; FELISBINO, SERGIO L.. Finasteride Inhibits Human Prostate Cancer Cell Invasion through MMP2 and MMP9 Downregulation. PLoS One, v. 8, n. 12, DEC 30 2013. (10/16671-3)