Control of P3HB-co-3HHx copolymer composition through gradative expression of phaA e phaB genes in Pseudomonas sp. LFM461 as host

• Grant number: 13/13495-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: January 01, 2014
• End date: February 29, 2016
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: José Gregório Cabrera Gomez
• Grantee: Lucas Garbini Cespedes
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 10/51692-1 - Metabolic engineering applied to biopolymer production, AP.BIOEN.JP
• Associated scholarship(s): 15/02058-1 - Evaluation of polyhydroxyalkanoates (PHA) production by Pseudomonas using a composition control plasmid (PCC) utilizing lignin as carbon source, BE.EP.MS

Abstract

Polyhydroxyalkanoates (PHA) are polyesters accumulated by bacteria and presents thermoplastic, biodegradable and biocompatible characteristics, therefore they can be produced from renewable raw materials. The copolymer 3-hydroxybutirate and 3-hydroxyhexanoate (P3HB-co-3HHx) tend to show increased elastic proprieties as lower 3HB monomer content, and more brittle as higher this content. This project aims the construction of a genetic system which allow the control of copolymer P3HB-co-3HHx through induction of genes responsible for 3HB precursor biosynthesis. In order to achieve that, a non-producing PHA Pseudomonas sp. LFM461 strain hosting different plasmids with regulated promoters will be constructed. The phaA and phaB genes will be under control of the different promoters and evaluated in efficiency, while the phaC gene will be expressed constitutively. It's expected that different levels of induction and the type of the promoter itself will change the final copolymer composition. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CESPEDES, Lucas Garbini. Composition control of P3HB-co-3HHx through gradative expression of phaA and phaB genes in Pseudomonas sp. LFM461.. 2016. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.