Metabolic engineering applied to biopolymer production

• Grant number: 10/51692-1
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
• Start date: March 01, 2011
• End date: February 29, 2016
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Marilda Keico Taciro
• Grantee: Marilda Keico Taciro
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 14/08061-1 - Genomic scale metabolic network reconstruction and improvement of polyhydroxyalkanoates production in Pseudomonas sp. (LFM046), BP.PD; 13/13495-8 - Control of P3HB-co-3HHx copolymer composition through gradative expression of phaA e phaB genes in Pseudomonas sp. LFM461 as host, BP.MS; 11/06645-8 - Metabolic engineering applied to biopolymer production, BP.JP.BIOEN

Abstract

Metabolic flux analysis and other tools of metabolic and biochemical engineering will be utilized for the improvement of the poly-3-hydroxybutyrate-co-3-hydroxyalkanoate of medium-chain-Iength [P(3HB-co-HAmcl)] production. Flux analysis will be performed using a free access software and experimental data generated in bioreactor. The study of essential nutrients limitation to microbial growth, to induce accumulation of the target product, will be held in a continuous mode operation, employing the technique of multiple nutrients limitations. Metabolic models will be proposed, simulated and validated utilizing the experimental data from bioreactor cultivations. The analysis of the metabolic models will provide information for genetic modifications and process strategies, in order to obtain higher yields and productivities. It is expected that, by the end of this project, the metabolic engineering approach emphasizing the integration of different methodologies and procedures shall be established. As a result, a Metabolic Engineering Group will be created, in such a way that, processes involving others bioproducts could be approached in a similar manner. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA-SILVA, LUIZIANA; TACIRO, MARILDA KEICO; RAICHER, GIL; MONIZ PICCOLI, ROSANE APARECIDA; MENDONCA, THATIANE TEIXEIRA; GARCEZ LOPES, MATEUS SCHREINER; CABRERA GOMEZ, JOSE GREGORIO. Perspectives on the production of polyhydroxyalkanoates in biorefineries associated with the production of sugar and ethanol. International Journal of Biological Macromolecules, v. 71, n. SI, p. 2-7, NOV 2014. (10/51989-4, 10/51692-1)

CARDINALI-REZENDE, JULIANA; PEREIRA DE SOUZA NAHAT, RAFAEL AUGUSTO TEODORO; GUZMAN MORENO, CESAR WILBER; CARRENO FARFAN, CARMEN ROSA; SILVA, LUIZIANA FERREIRA; TACIRO, MARILDA KEICO; CABRERA GOMEZ, JOSE GREGERIO. Draft Genome Sequence of Halomonas sp. HG01, a Polyhydroxyalkanoate-Accumulating Strain Isolated from Peru. GENOME ANNOUNCEMENTS, v. 4, n. 1, p. 2-pg., 2016-01-01. (10/51692-1)

FERREIRA-SILVA, LUIZIANA; TACIRO, MARILDA KEICO; RAICHER, GIL; MONIZ PICCOLI, ROSANE APARECIDA; MENDONCA, THATIANE TEIXEIRA; GARCEZ LOPES, MATEUS SCHREINER; CABRERA GOMEZ, JOSE GREGORIO. Perspectives on the production of polyhydroxyalkanoates in biorefineries associated with the production of sugar and ethanol. International Journal of Biological Macromolecules, v. 71, p. 6-pg., 2014-11-01. (10/51692-1, 10/51989-4)

CARDINALI-REZENDE, JULIANA; PEREIRA DE SOUZA NAHAT, RAFAEL AUGUSTO TEODORO; GUZMAN MORENO, CESAR WILBER; CARRENO FARFAN, CARMEN ROSA; SILVA, LUIZIANA FERREIRA; TACIRO, MARILDA KEICO; CABRERA GOMEZ, JOSE GREGERIO. Draft Genome Sequence of Halomonas sp. HG01, a Polyhydroxyalkanoate-Accumulating Strain Isolated from Peru. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 4, n. 1, JAN-FEB 2016. (10/51692-1)

CARDINALI-REZENDE, JULIANA; RADUAN ALEXANDRINO, PAULO MOISES; PEREIRA DE SOUZA NAHAT, RAFAEL AUGUSTO THEODORO; VIEIRA SANT'ANA, DEBORA PARRINE; SILVA, LUIZIANA FERREIRA; CABRERA GOMEZ, JOSE GREGORIO; TACIRO, MARILDA KEICO. Draft Genome Sequence of Pseudomonas sp. Strain LFM046, a Producer of Medium-Chain-Length Polyhydroxyalkanoate. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 3, n. 4, JUL-AUG 2015. (10/51692-1, 13/50357-2, 14/08061-1)

CESPEDES, LUCAS G.; NAHAT, RAFAEL A. T. P. S.; MENDONCA, THATIANE T.; TAVARES, RAFAELA R.; OLIVEIRA-FILHO, EDMAR R.; SILVA, LUIZIANA F.; TACIRO, MARILDA K.; SANCHEZ, RUBEN J.; GOMEZ, JOSE G. C.. A non-naturally-occurring P(3HB-co-3HA(MCL)) is produced by recombinant Pseudomonas sp from an unrelated carbon source. International Journal of Biological Macromolecules, v. 114, p. 512-519, JUL 15 2018. (10/51692-1, 14/15448-0)

CARDINALI-REZENDE, JULIANA; RADUAN ALEXANDRINO, PAULO MOISES; PEREIRA DE SOUZA NAHAT, RAFAEL AUGUSTO THEODORO; VIEIRA SANT'ANA, DEBORA PARRINE; SILVA, LUIZIANA FERREIRA; CABRERA GOMEZ, JOSE GREGORIO; TACIRO, MARILDA KEICO. Draft Genome Sequence of Pseudomonas sp. Strain LFM046, a Producer of Medium-Chain-Length Polyhydroxyalkanoate. GENOME ANNOUNCEMENTS, v. 3, n. 4, p. 2-pg., 2015-07-01. (10/51692-1, 13/50357-2, 14/08061-1)

CARDINALI-REZENDE, JULIANA; DI GENOVA, ALEX; NAHAT, RAFAEL A. T. P. S.; STEINBUECHEL, ALEXANDER; SAGOT, MARIE-FRANCE; COSTA, RAFAEL S.; OLIVEIRA, HENRIQUE C.; TACIRO, MARILDA K.; SILVA, LUIZIANA F.; GOMEZ, JOSE GREGORIO C.. The relevance of enzyme specificity for coenzymes and the presence of 6-phosphogluconate dehydrogenase for polyhydroxyalkanoates production in the metabolism of Pseudomonas sp. LFM046. International Journal of Biological Macromolecules, v. 163, p. 240-250, NOV 15 2020. (10/51692-1, 14/08061-1)

OLIVEIRA-FILHO, EDMAR RAMOS; GUAMAN, LINDA P.; MENDONCA, THATIANE TEIXEIRA; LONG, PAUL F.; TACIRO, MARILDA KEICO; CABRERA GOMEZ, JOSE GREGORIO; SILVA, LUIZIANA F.. Production of Polyhydroxyalkanoates Copolymers by Recombinant Pseudomonas in Plasmid- and Antibiotic-Free Cultures. JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY, v. 28, n. 5, p. 225-235, 2018. (12/51533-6, 10/51692-1, 14/15448-0)