Effect of smoking on growth, viability, biofilm formation and gene expression of Streptococcus sanguinis

• Grant number: 13/02302-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2014
• End date: July 31, 2015
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Priscila Larcher Longo
• Grantee: Paola Gabriela Buonafina Pereira
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Smoking affects the body homeostasis at various levels. The habit is connected to oral diseases, such as periodontitis, by negatively affect the host's immune system and to influence expression of virulence-associated genes in microorganisms periodontal pathogens. However, there is few reports about the influence of smoking on oral commensal microbiota. Objectives: To analyze the effect of cigarette smoke extract on cultures of oral commensal organism Streptococcus sanguinis. Materials and Methods: The effect of smoking on S. sanguinis will be assessed using the soluble components of cigarette smoke extract on microbial growth in culture, cell viability, ability to biofilm formation and expression of genes associated with virulence and IgA1 NT5.