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Development of hexamethyldisiloxane films incorporated with chlorhexidine onto titanium surface: antimicrobial potential and cytotoxicity

Grant number: 16/06117-5
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2016
Effective date (End): February 29, 2020
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal researcher:Valentim Adelino Ricardo Barão
Grantee:Adaias Oliveira Matos
Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Associated scholarship(s):17/21894-0 - Antimicrobial and biocompatibility potentials of hexamethyldisiloxane films incorporated with chlorhexidine on titanium surface using a co-infected in vitro reconstituted gingival oral epithelium model, BE.EP.DR


A stable connection between the implant titanium surface/abutment and periimplant soft tissues is an important approach for the long-term success of dental implant. Thus, such surface should minimize the bacterial colonization while allowing a good adhesion of the oral tissues. Therefore, the objective of this research project is to develop hexamethyldisiloxane films incorporated with different concentrations of chlorhexidine (CHX) (0 - control, 0.35, 0.70 and 1.40% - w/w) onto commercially pure titanium (cpTi) surface and to evaluate their antimicrobial potential and cytotoxicity. Machined surface will also be used as control. Discs surfaces will be characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), atomic force microscopy (AFM), x-ray diffraction analysis (XRD), surface roughness, Raman spectroscopy, film thickness, wettability and surface free energy. In the microbiological assay, biofilm of Streptococcus sanguinis will grow on discs surfaces during 2 hours (biofilm adhesion) and 48 hours (mature biofilm). The colony forming units will be determined and confocal laser scanning will be used to investigate the biofilm structure. High performance liquid chromatography analysis is used to quantify the amount of drug released as a function of time. Another microbiological assay will be performed after stabilization of chlorhexidine release. For the cytotoxicity assay cultures of fibroblasts will be used and the cell morphology and viability, genes and collagen expressions will be determined. Quantitative data will be statistically analyzed at a significant level of 5%. The number of samples for each assay will be determined by a pilot study.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PANTAROTO, HELOISA NAVARRO; DE ALMEIDA, AMANDA B.; GOMES, ORISSON P.; MATOS, ADAIAS O.; LANDERS, RICHARD; CASARIN, RENATO CORREA V.; DA SILVA, JOSE HUMBERTO D.; NOCITI, JR., FRANCISCO H.; BARAO, VALENTIM A. R.. Outlining cell interaction and inflammatory cytokines on UV-photofunctionalized mixed-phase TiO2 thin film. Materials Science & Engineering C-Materials for Biological Applications, v. 118, . (16/06117-5, 16/11470-6)
MATOS, ADAIAS OLIVEIRA; DE ALMEIDA, AMANDA BANDEIRA; BELINE, THAMARA; TONON, CAROLINE C.; VIANA CASARIN, RENATO CORREA; WINDSOR, LESTER JACK; DUARTE, SIMONE; NOCITI JR, FRANCISCO HUMBERTO; RANGEL, ELIDIANE CIPRIANO; GREGORY, RICHARD L.; et al. Synthesis of multifunctional chlorhexidine-doped thin films for titanium-based implant materials. Materials Science & Engineering C-Materials for Biological Applications, v. 117, . (16/06117-5, 17/21894-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MATOS, Adaias Oliveira. Antimicriobial potential and cytotoxicity of hexamethyldisiloxane film incorporated with chlorhexidine on the titanium surface. 2020. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.

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Filed patent(s) as a result of this research project

Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação BR1020180148710 - Universidade Estadual de Campinas (UNICAMP) ; Universidade Estadual Paulista (UNESP) . Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação - July 2018, 20