Mining Streptomyces sp. B1 metabolites: isolation and characterization
| Grant number: | 13/16037-0 |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| Effective date (Start): | January 01, 2014 |
| Effective date (End): | December 31, 2017 |
| Field of knowledge: | Biological Sciences - Microbiology |
| Principal Investigator: | Itamar Soares de Melo |
| Grantee: | Suikinai Nobre Santos |
| Home Institution: | Embrapa Meio-Ambiente. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). Jaguariúna , SP, Brazil |
| Associated scholarship(s): | 14/24556-0 - Determination of gene clusters involved in the biosynthetic pathway for phenazine antibiotic and assembly of biosynthetic pathways correlated in a new species of Streptomyces (CCMA/Caat52) isolated from the Caatinga biome of Brazil, BE.EP.PD |
Abstract Currently, cancer is a major disease causing death or cause irreparable damage. The recent advances in sequence of microbial genomes has substantially changed our understanding of the metabolic capabilities of microorganisms. Ecosystems in tropical regions such as Caatinga, dry forest from Brazil, has been targeted search of natural compounds because of this unique environment resulting in a wealth of highly adapted species. Thus, enzymes and other bioactive metabolites produced by these microorganisms have the potential to have unique physiological and biochemical properties. Preliminary studies with micro-organisms isolated from Caatinga and identified the Streptomyces sp. CCMA168 strain noted great potential in the production of antitumor molecules. This work aims to identify genes (PKS and NRPS) biosynthetic pathways responsible for production of the anticancer compounds from Streptomyces sp CCMA168 strain, also propose experimental models generated mutants defective and producers of the target molecule as tactic for to know the production of compounds. Tools of genomics and metabolomics are used of strategies for completion of work. (Step 1) obtaining the transformed strains by random mutation using transposon Tn5-specific in Streptomyces, the signaling pathways involved in biosynthesis of the PKS and NRPS; (Stage II) monitoring suppression and/or production of anticancer compounds obtained in turning; (Stage III) identification and characterization of genes changed; (Stage IV) identification and structural elucidation of the active molecule (Stage V) sequencing all genome of this Streptomyces and polyphasic taxonomy studies to complete identification. | |