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Isolation and characterization of genes correlated to biosynthesis of antitumor molecules in Streptomyces SP

Grant number: 13/16037-0
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): January 01, 2014
Effective date (End): December 31, 2017
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Itamar Soares de Melo
Grantee:Suikinai Nobre Santos
Home Institution: Embrapa Meio-Ambiente. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). Jaguariúna , SP, Brazil
Associated scholarship(s):14/24556-0 - Determination of gene clusters involved in the biosynthetic pathway for phenazine antibiotic and assembly of biosynthetic pathways correlated in a new species of Streptomyces (CCMA/Caat52) isolated from the Caatinga biome of Brazil, BE.EP.PD

Abstract

Currently, cancer is a major disease causing death or cause irreparable damage. The recent advances in sequence of microbial genomes has substantially changed our understanding of the metabolic capabilities of microorganisms. Ecosystems in tropical regions such as Caatinga, dry forest from Brazil, has been targeted search of natural compounds because of this unique environment resulting in a wealth of highly adapted species. Thus, enzymes and other bioactive metabolites produced by these microorganisms have the potential to have unique physiological and biochemical properties. Preliminary studies with micro-organisms isolated from Caatinga and identified the Streptomyces sp. CCMA168 strain noted great potential in the production of antitumor molecules. This work aims to identify genes (PKS and NRPS) biosynthetic pathways responsible for production of the anticancer compounds from Streptomyces sp CCMA168 strain, also propose experimental models generated mutants defective and producers of the target molecule as tactic for to know the production of compounds. Tools of genomics and metabolomics are used of strategies for completion of work. (Step 1) obtaining the transformed strains by random mutation using transposon Tn5-specific in Streptomyces, the signaling pathways involved in biosynthesis of the PKS and NRPS; (Stage II) monitoring suppression and/or production of anticancer compounds obtained in turning; (Stage III) identification and characterization of genes changed; (Stage IV) identification and structural elucidation of the active molecule (Stage V) sequencing all genome of this Streptomyces and polyphasic taxonomy studies to complete identification.

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VARGAS HOYOS, HAROLD ALEXANDER; SANTOS, SUIKINAI NOBRE; DA SILVA, LEONARDO JOSE; PAULINO SILVA, FABIO SERGIO; GENUARIO, DIEGO BONADO; ZUCCHI, TIAGO DOMINGUES; MELO, ITAMAR SOARES. Streptomyces rhizosphaericola sp. nov., an actinobacterium isolated from the wheat rhizosphere. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, v. 69, n. 8, p. 2431-2439, AUG 2019. Web of Science Citations: 0.
VASCONCELLOS, RAFAEL L. F.; SANTOS, SUIKINAI NOBRE; ZUCCHI, TIAGO DOMINGUES; SILVA, FABIO SERGIO PAULINO; SOUZA, DANILO TOSTA; MELO, ITAMAR SOARES. Pseudomonas aestus sp nov., a plant growth-promoting bacterium isolated from mangrove sediments. Archives of Microbiology, v. 199, n. 8, p. 1223-1229, OCT 2017. Web of Science Citations: 1.
KAVAMURA, VANESSA NESSNER; SANTOS, SUIKINAI NOBRE; TAKETANI, RODRIGO GOUVEA; FIGUEIREDO VASCONCELLOS, RAFAEL LEANDRO; MELO, ITAMAR SOARES. Draft Genome Sequence of Plant Growth-Promoting Drought-Tolerant Bacillus sp. Strain CMAA 1363 Isolated from the Brazilian Caatinga Biome. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 5, n. 5 FEB 2017. Web of Science Citations: 0.
SANTOS, SUIKINAI NOBRE; KAVAMURA, VANESSA NESSNER; TAKETANI, RODRIGO GOUVEA; VASCONCELLOS, RAFAEL L. F.; ZUCCHI, TIAGO DOMINGUES; MELO, ITAMAR SOARES. Draft Genome Sequence of Bacillus sp. Strain CMAA 1185, a Cellullolytic Bacterium Isolated from Stain House Lake, Antarctic Peninsula. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 3, n. 3 MAY-JUN 2015. Web of Science Citations: 1.

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