Expression of the secondary route of glucose uptake in canine corpus luteum

• Grant number: 13/22405-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2014
• End date: December 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Paula de Carvalho Papa Keohane
• Grantee: Loren D Aprile
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The diestrus is the luteal phase in the bitch characterized by increased progesterone (P4) serum the first half and fluctuations of 17²-estradiol (E2) the second half. The corpus luteum (CL) is a temporary endocrine, passing through a development process, maintenance and regression, reaching full secretory activity when their training is complete. Insulin is anabolic hormone essential for maintenance of glucose homeostasis, growth and cell differentiation, being secreted by pancreatic ² cells. As the CL has regular periods of training, activity and regression marked by intense tissue remodeling, a supply of glucose suitable for luteal cells must be ensured. Therefore, this study aims to evaluate the spatio-temporal expression of genes participating in the sideline of glucose uptake Cbl-CAP (Cbl, CAP, CrK II, C3G e TC10) in CL throughout diestrus bitches, correlate this expression with serum insulin, P4 e E2. For this, samples of corpora lutea bitches every 10 days between days 10 and 60 diestrus will be collected for analysis by immunohistochemistry, protein expression and by real time PCR the gene expression of the constituents of the byways of glucose uptake. In addition, blood samples will be collected for measurement of steroid hormones and insulin. The data will be analyzed for normality and will be tested by ANOVA when there is normal distribution or Kruskal-Wallis otherwise. The level of significance is p <0.05.