Use of probiotics and essential oils in the diet on the development of the intestinal mucosa in broiler

• Grant number: 13/18629-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2014
• End date: January 31, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Renato Luis Furlan
• Grantee: Gabriela Gomes de Freitas
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

This work will be carried out to evaluate the effects of adding Bacillus subtilis (probiotic), a commercial mixture of essential oils or a combination of both in the diet on the performance and intestinal morphology in the small intestine of chickens. For this, we used 990 broiler chickens of Cobb-500 ®, distributed in a completely randomized design with five treatments and six replicates of 33 birds each. The treatments consisted of: control diet (CD), DC + 10 ppm Avilamycin (ANTI), DC + essential oil 120 g / 1000 kg of feed (OE), DC + Bacillus subtilis 500 g / 1000 kg ration (PRO) , DC + 80 g essential oil + 500 g Bacillus subtilis / 1000 kg of feed (OE + PRO). At 7, 21 and 42 days of age was obtained for zootechnical performance data and samples were collected approximately 10 cm from the middle portion of the jejunum, through repetition of a bird to assess the following parameters: villus height, depth crypts and goblet cell number. The data will be submitted to analysis of variance by the General Linear Model (GLM) of SAS ® (SAS Institute, 2002) and in case of a statistically significant difference (p <0.05), the averages are compared by Tukey test at 5% probability.