Use of probiotics and essential oil in the diet on the intestinal microbiota, the activity of digestive enzymes and the expression of genes related to digestion and absorption of nutrients in chickens

• Grant number: 12/03927-5
• Support Opportunities: Regular Research Grants
• Duration: June 01, 2012 - July 31, 2014
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Production
• Principal Investigator: Renato Luis Furlan
• Grantee: Renato Luis Furlan
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated researchers: João Martins Pizauro Junior ; Marcos Macari

Abstract

The use of antibiotic growth promoters in poultry diets has contributed markedly to the improvement of production rates in the industry, worldwide. However, its continued use in the diet for decades has brought problems like the emergence of bacteria resistant to antibiotic, residues in poultry products and the environment and, imbalance in the normal microbiota of birds. Among the possible alternatives to the question, the use of probiotics and essential oils has shown positive results, such as modulation of intestinal microbiota, improving intestinal health, digestibility and bird performance. However, some trials have reported limited effects of both products. Thus, the study of the responses performed by these additives in molecular aspects related to the processes of digestion and absorption of birds as well as the intestinal colonization by bacteria of importance to the host, may contribute to its best use in animal diets. Based on these, this paper proposes to assess the effects of the addition of Bacillus subtilis (probiotic), a commercial mixture of essential oils or a combination of both on performance, abundance of 16S rDNA sequences of phylogenetic groups from the intestinal microbiota [Lactobacillus (Lactobacillus aviarius, Lactobacillus salivarius), Enterobacteriaceae, Enterococcus, and Clostridium perfringens], the activity of digestive enzymes intestinal (aminopeptidase, maltase and sucrase) and ATPase Na+/K+, and the expression of some key genes related to digestion processes [aminopeptidase N (APN), sucrose-isomaltase (SI), maltase-glucoamylase (MGAM)] and absorption of nutrients [intestinal peptide transporter 1 (PEPT-1), glucose cotransporter (SGLT-1), glucose transporter 2 (GLUT-2) and ATPase and Na+/K+] in broilers. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ANTONIO, J.; FERNANDEZ-ALARCON, M. F.; LUNEDO, R.; SQUASSONI, G. H.; FERRAZ, A. L. J.; MACARI, M.; FURLAN, R. L.; FURLAN, L. R.. Chronic heat stress and feed restriction affects carcass composition and the expression of genes involved in the control of fat deposition in broilers. JOURNAL OF AGRICULTURAL SCIENCE, v. 155, n. 9, p. 1487-1496, NOV 2017. (12/03927-5)

FERNANDEZ-ALARCON, M. F.; TROTTIER, N.; STEIBEL, J. P.; LUNEDO, R.; CAMPOS, D. M. B.; SANTANA, A. M.; PIZAURO, JR., J. M.; FURLAN, R. L.; FURLAN, L. R.. Interference of age and supplementation of direct-fed microbial and essential oil in the activity of digestive enzymes and expression of genes related to transport and digestion of carbohydrates and proteins in the small intestine of broilers. POULTRY SCIENCE, v. 96, n. 8, p. 2920-2930, AUG 2017. (12/03927-5, 11/17378-0)