Genetic characterisation of Aedes fluviatilis populations from municipal parks in São Paulo, using microsatellite markers

• Grant number: 13/22347-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2014
• End date: February 29, 2016
• Field of knowledge: Biological Sciences - Parasitology - Entomology and Malacology of Parasites and Vectors
• Principal Investigator: Mauro Toledo Marrelli
• Grantee: Laura Cristina Multini
• Host Institution: Instituto de Medicina Tropical de São Paulo (IMT). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Aedes fluviatilis is a species of mosquito highly anthropophilic and well adapted to the urban environment. This species was found in abundance in municipal parks in the city of São Paulo. Although this species has not been implicated in the transmission of infections to humans under natural conditions, several studies have shown its vector competence for the yellow fever virus, Plasmodium gallinaceum and Dirofilaria immitis. Microsatellites are polymorphic markers consisting of non-coding regions of DNA with 1 to 6 pairs of bases repeated in tandem. Because they have high rate of mutation and be neutral markers of high variability, the microsatellites are widely used in population studies. Whereas Ae. fluviatilis is a species abundant in the city of São Paulo, with epidemiological interest and scarce information on his bio-ecology and genetic structure, the objectives of this project are: to assess the genetic variability and the gene flow between populations of the mosquito Ae. fluviatilis in São Paulo; to test parameters of microsatellite markers feature in Ae. fluviatilis, previously used successfully in other species of culicidae. It is of great importance to assess the genetic structure of populations of Ae. fluviatilis for a better understanding of their potential epidemiological.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MULTINI, LAURA CRISTINA; BRUNO WILKE, ANDRE BARRETTO; SUESDEK, LINCOLN; MARRELLI, MAURO TOLEDO. Population Genetic Structure of Aedes fluviatilis (Diptera: Culicidae). PLoS One, v. 11, n. 9, SEP 6 2016. (13/15313-4, 12/19117-2, 13/22347-2)

MULTINI, LAURA CRISTINA; MARRELLI, MAURO TOLEDO; BRUNO WILKE, ANDRE BARRETTO. Microsatellite loci cross-species transferability in Aedes fluviatilis (Diptera:Culicidae): a cost-effective approach for population genetics studies. PARASITES & VECTORS, v. 8, DEC 15 2015. (12/19117-2, 13/15313-4, 13/22347-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MULTINI, Laura Cristina. Genetic characterization of Aedes fluviatilis populations from municipal parks in São Paulo, using microsatellite markers. 2016. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Medicina Tropical de São Paulo (IMT) São Paulo.