Patterns of emergence of multidrug resistant enterococci and staphylococci in Brazil and search for new drugs

• Grant number: 13/24952-0
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): March 01, 2014
• Effective date (End): June 30, 2018
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal researcher: Ilana Lopes Baratella da Cunha Camargo
• Grantee: Andrei Nicoli Gebieluca Dabul Dias de Sousa
• Home Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery, AP.CEPID

Abstract

Enterococci and staphylococci are important nosocomial pathogens, and among them stand out Vancomycin-resistant Enterococcus sp. (VRE) and methicillin-resistant S. aureus (MRSA). During a study with VRE and MRSA isolated in 2009 and 2011 on Hospital Risoleta Tolentino Neves, in Belo Horizonte, it was detected resistance to tigecycline emerging in E. faecalis and S. aureus. E. faecalis isolated showing resistence to this glycylcycline are ST103 and belong to the same pulsotype. S. aureus are ST105-SCCmecII. Thus, the aim of this project is to use the next-generation sequencing technology to evaluate the emergence and spread of tigecycline resistance determinants on these lineages. Besides, it will be tested natural products on these lineages aiming the bacterial cell death, inhibition of the bacterial growth, or biofilm degradation. Furthermore, the detailed study on MRSA genes where possibly will be encountered mutations related to the tigecycline resistance process is also an objective. It will be done through microbiology and molecular biology techniques. Few studies have had the aim to elucidate the tigecycline resistance mechanism in Gram-positives, which is why the opportunity to study something novel on a Brazilian institution is so important, even more because it is where the study started.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA, ROSINEIDE CARDOSO; GEBIELUCA DABUL, ANDREI NICOLI; DOS SANTOS BORALLI, CAMILA MARIA; ZUVANOV, LUIZA; BARATELLA DA CUNHA CAMARGO, ILANA LOPES. Dissemination of bla(KPC-2) in an NTEKPC by an IncX5 plasmid. PLASMID, v. 106, NOV 2019. Web of Science Citations: 0.

GEBIELUCA DABUL, ANDREI NICOLI; AVACA-CRUSCA, JULIANA SPOSTO; NAVAIS, ROBERTO BARRANCO; MERLO, THAIS PANHAN; VAN TYNE, DARIA; GILMORE, MICHAEL S.; BARATELLA DA CUNHA CAMARGO, ILANA LOPES. Molecular basis for the emergence of a new hospital endemic tigecycline-resistant Enterococcus faecalis ST103 lineage. INFECTION GENETICS AND EVOLUTION, v. 67, p. 23-32, JAN 2019. Web of Science Citations: 1.

OKADO, JESSICA BALEIRO; AVACA-CRUSCA, JULIANA SPOSTO; OLIVEIRA, ALEXANDRE LIMA; GEBIELUCA DABUL, ANDREI NICOLI; BARATELLA DA CUNHA CAMARGO, ILANA LOPES. Daptomycin and vancomycin heteroresistance revealed among CC5-SCCmecII MRSA clone and in vitro evaluation of treatment alternatives. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 14, p. 209-216, SEP 2018. Web of Science Citations: 3.

GEBIELUCA DABUL, ANDREI NICOLI; AVACA-CRUSCA, JULIANA SPOSTO; VAN TYNE, DARIA; GILMORE, MICHAEL S.; BARATELLA CUNHA CAMARGO, ILANA LOPES. Resistance in In Vitro Selected Tigecycline-Resistant Methicillin-Resistant Staphylococcus aureus Sequence Type 5 Is Driven by Mutations in mepR and mepA Genes. MICROBIAL DRUG RESISTANCE, v. 24, n. 5, p. 519-526, JUN 2018. Web of Science Citations: 9.

DE MELLO, SUELEN SCARPA; VAN TYNE, DARIA; GEBIELUCA DABUL, ANDREI NICOLI; GILMORE, MICHAEL S.; CAMARGO, ILANA L. B. C. High-Quality Draft Genome Sequence of the Multidrug-Resistant Clinical Isolate Enterococcus faecium VRE16. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 4, n. 5 SEP-OCT 2016. Web of Science Citations: 1.