Enterococci and staphylococci are important nosocomial pathogens, and among them stand out Vancomycin-resistant Enterococcus sp. (VRE) and methicillin-resistant S. aureus (MRSA). During a study with VRE and MRSA isolated in 2009 and 2011 on Hospital Risoleta Tolentino Neves, in Belo Horizonte, it was detected resistance to tigecycline emerging in E. faecalis and S. aureus. E. faecalis isolated showing resistence to this glycylcycline are ST103 and belong to the same pulsotype. S. aureus are ST105-SCCmecII. Thus, the aim of this project is to use the next-generation sequencing technology to evaluate the emergence and spread of tigecycline resistance determinants on these lineages. Besides, it will be tested natural products on these lineages aiming the bacterial cell death, inhibition of the bacterial growth, or biofilm degradation. Furthermore, the detailed study on MRSA genes where possibly will be encountered mutations related to the tigecycline resistance process is also an objective. It will be done through microbiology and molecular biology techniques. Few studies have had the aim to elucidate the tigecycline resistance mechanism in Gram-positives, which is why the opportunity to study something novel on a Brazilian institution is so important, even more because it is where the study started.
News published in Agência FAPESP Newsletter about the scholarship: