Effect of the low level laser irradiation (830nm) on the implants osseointegration in grafted areas with different osteoconductors biomaterials

• Grant number: 13/24318-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: April 01, 2014
• End date: March 31, 2017
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Rosemary Adriana Chierici Marcantonio
• Grantee: Guilherme José Pimentel Lopes de Oliveira
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The aim of this project is to evaluate the effect of irradiation with low intensity laser (GaAlAs - 830nm) on the integration of biomaterials (B) of the xenogenic (EX) and alloplastic origin (EA), and osseointegration of implants (I) in these regions. For this purpose, this project will be divide into two studies. In the study 1it will assess the effect of irradiation with low intensity laser on the integration of the xenogenic and alloplastic graft on the receptor site. 90 rats will be use and these animals will be allocate into 6 groups with 15 animals each : G1 : No biomaterial; G2 : No biomaterial + laser ; G3 : Deproteinized bovine bone (DBB) ; G4 : DBB + Laser irradiation; G5 : ²-tricalcium phosphate / hydroxyapatite (²TCP/HA); G6 : ²TCP/HA + laser . The animals will be euthanize after 30, 60 and 90 days of the surgeries. It will be perform micro-tomographic, histomorphometric, immunohistochemical and qPCR analysis. In the study 2 it will be evaluate the effect of low intensity laser irradiation on the osseointegration of dental implants in grafted areas. It will be use 240 rats that will be randomly allocate into 8 groups with 30 animals in each: G1: DBB; G2 : DBB + laser (on biomaterial and implants); G3 : DBB+ Laser (only on biomaterial); G4 : DBB + laser (only on implants); G5 : ²TCP/HA; G6 : ²TCP/HA + laser on biomaterial and implants); G7 : ²TCP/HA +Laser (only on biomaterial); G8 : ²TCP/HA + laser (only on implants). The implants will be insert after 60 or 120 days of the insertion of biomaterials. The animals will be euthanize 15, 60 and 90 days after the implant placement. It will be perform biomechanical, micro-tomographic, histomorphometric, and immunohistochemical analysis.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, GUILHERME J. P. L.; ARONI, MAURICIO A. T.; MEDEIROS, MARCELL C.; MARCANTONIO, JR., ELCIO; MARCANTONIO, ROSEMARY A. C.. Effect of low-level laser therapy on the healing of sites grafted with coagulum, deproteinized bovine bone, and biphasic ceramic made of hydroxyapatite and -tricalcium phosphate. In vivo study in rats. Lasers in Surgery and Medicine, v. 50, n. 6, p. 651-660, AUG 2018. (13/24318-0)

PIMENTEL LOPES DE OLIVEIRA, GUILHERME JOSE; TINAJERO ARONI, MAURICIO ANDRES; PINOTTI, FELIPE EDUARDO; MARCANTONIO JR, ELCIO; CHIERICI MARCANTONIO, ROSEMARY ADRIANA. Low-level laser therapy (LLLT) in sites grafted with osteoconductive bone substitutes improves osseointegration. Lasers in Medical Science, v. 35, n. 7, FEB 2020. (13/24318-0)

GUILHERME JOSÉ PIMENTEL LOPES DE OLIVEIRA; FELIPE EDUARDO PINOTTI; MAURÍCIO ANDRES TINAJERO ARONI; ELCIO MARCANTONIO JR; ROSEMARY ADRIANA CHIÉRICI MARCANTONIO. Effect of different low-level intensity laser therapy (LLLT) irradiation protocols on the osseointegration of implants placed in grafted areas. Journal of Applied Oral Science, v. 29, 2021-04-14. (13/24318-0)